Shotgun cloning

BASIC GENE CLONING Shotgun cloning experiment... 

Shotgunning Cloning of a complete set of DNA fragments from a particular genome. 

Rapid random sequencing by shotgun cloning into single stranded phage vectors (Sanger et al., 1980)... 

In the shotgun sequencing procedure picking recombinant clones is a random procedure and it is often convenient, having sequenced from one end of a single-stranded insert, to turn the sequence around to be able to sequence from the other end. This is done using the clone tum-around procedure described by Winter... 

These general approaches of course may be modified or restructured to suit a particular problem. In probably the majority of sequencing undertakings the DNA of interest will have been cloned and amplified in a suitable vector and while in some instances the cloned fragment can be precisely cleaved out from the recombinant vector and isolated by gel electrophoresis, in other cases this may not be possible. The shotgun method described above will yield a complex array of end-labelled fragments and the problem immediately arises of distinguishing which ones... 

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