Shotgun method

In some rare cases, the shotgun method appears to be the only feasible one. A typical example is the cyclodehydrooligomerization of diethynylmethano-... 

Synthesis of FK506-Afflnlty Resins by Nonselective Immobilization Without Specific Skills ( Chemical Shotgun Method)... 

The identification of the lipids is a very challenging task. The lack of comprehensive mass spectral libraries often limits the identification of compounds in LC-MS and shotgun methods. Some spectral libraries are available, such as the Human Metabolome Database (http /www.hmdb.ca), the METLIN Metabolite Database (http /metlin.scripps.edu) (24), and the MassBank (http /www. massbank.jp) (25). However, construction of universal spectral databases for API-MS is challenging due to the poor reproducibility and high interinstru-ment variability of fragmentation patterns. 

These general approaches of course may be modified or restructured to suit a particular problem. In probably the majority of sequencing undertakings the DNA of interest will have been cloned and amplified in a suitable vector and while in some instances the cloned fragment can be precisely cleaved out from the recombinant vector and isolated by gel electrophoresis, in other cases this may not be possible. The shotgun method described above will yield a complex array of end-labelled fragments and the problem immediately arises of distinguishing which ones... 

Staden, R. (1982) Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Res. 10, 4731-4751. 

Wu F, Sun D, Wang N, et al. Comparison of surfaetant-assisted shotgun methods using acid-labile surfactants and sodium dodecyl sulfate for membrane proteome analysis. Anal Chim Acta. 2011 698 36 3. doi 10.1016/j.aca.2011.04.039. 

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