Cloning oxidizing enzymes

Three isoforms of NO synthesizing enzymes ( nitric oxide synthase (NOS)) were isolated, purified, and cloned neuronal NO synthase ( neuronal nitric oxide synthase (nNOS) or isoform (I), immunological or inducible NOS ( inducible (immunological) nitric oxide synthase (iNOS) or isoform (II), and endothelial NOS ( endothelial nitric oxide synthase (eNOS) or isoform... 

Parallel to the modification of the catalytic performance in Baeyer-Villiger oxidations, random mutagenesis was successfully applied to improve the stereoselectivity of CHMOAcineto hi cascs of essentially racemic sulfoxide formation. In addition, enantiodivergent clones with >98% ee for both antipodal products were identified (Table 9.5) [205]. However, improvement in stereoselectivity of mutant enzymes was often accompanied by increased formation of sulfone. This effect can also be utilized to resolve racemic sulfoxides. 

Sutherland TD, I Home, RJ Russell, JG Oakeshott (2002) Gene cloning and molecular characterization of a two-enzyme system catalyzing the oxidative detoxification of 3-endosulfan. Appl Environ Microbiol 68 6237-6245. 

LAUDERT, D., PFANNSCHMIDT, U., LOTTSPEICH, F HOLLANDER-CZYTKO, H., WEILER, E.W., Cloning, molecular and functional characterization of Arabidopsis thaliana allene oxide synthase (CYP74) the first enzyme for the octadecanoid pathway to jasmonates, Plant Mol. Biol., 1996,31,323-335. 

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