Cleland

In this case, the leading substrate, A (also called the obligatory or compulsory substrate), must bind first. Then the second substrate, B, binds. Strictly speaking, B cannot bind to free enzyme in the absence of A. Reaction between A and B occurs in the ternary complex, and is usually followed by an ordered release of the products of the reaction, P and Q. In the schemes below, Q is the product of A and is released last. One representation, suggested by W. W. Cleland, follows ... 

Gerlt, J. A., Kreevoy, M. M., Cleland, W. W., and Frey, P. A., 1997. Understanding enzymic catalysis The importance of short, strong hydrogen bonds. Chemistry and Biology 4 259-267. 

Winters, P. Cleland, F. Pietrzyk, M. Burris, M and Perez, R. (2000). Predicting Changes in Average Vehicle Ridership on the Basis of Employer Trip Reduction Plans. Transportation Research Record 1682. Transportation Research Board. Washington, DC National Research Council. 

Cosgrove, D.J. Cleland, R.E. (1983). Solutes in the free space of growing stem tissues. Plant Physiology, 73, 326-31. 

Van Volkenburgh, E. Cleland, R.E. (1986). Wall yield threshold and effective turgor of growing bean leaves. Planta, 167, 37-43. 

Dhindsa, R.S. Cleland, R.E. (1975). Water stress and protein synthesis. I. Differential inhibition of protein synthesis. Plant Physiology, 55, 778-81. 

Cleland, M.R. and Earrell, J.P., Proceedings of the Eourth Conference on AppUcation of SmaU Accelerators, North Texas State Univ, Denton, Texas, 1976. 

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