Chromogenic chemical detection reagents

Royo S, Martmez-Manez R, Sancenon F, Costero AM, Parra M, Gil S. Chromogenic and fluorogenic reagents for chemical warfare nerve agents detection. Chem Commun 2007 4839-47. 

Unfortunately, there are no universal methods to detect all types of protein oxidation, because the products formed can be so diverse in nature. However, some forms of protein oxidation can be assayed using chemical modification (Davies et al., 1999 Shacter, 2000). In particular, the formation of carbonyl groups on proteins can be targeted using the reagent 2,4-dinitrophenyl-hydrazine (DNPH). This compound reacts with aldehydes to form 2,4-dinitrophenylhydrazone derivatives, which create chromogenic modifications that can be detected at high sensitivity in microplate assays or Western blot analysis (Buss et al., 1997 Winterbourn et al., 1999). 

Richardson also devised many novel reagents and chromogenic substances that are now used in various diagnostic kits of value for the rapid and accurate visual detection of human disease, as well as for the identification of pathogenic organisms associated with disease. For chemical posterity, it is therefore considered important to present a detailed account of Richardson s numerous research contributions. 

BI SP The flow cell is filled by injecting in the flow system a homogeneous bead suspension of an appropriate solid support previously loaded with the chromogen ic reagent. The solid beads work as a flow-through chemical sensing microzone integrating online separation/reaction/ detection 0.02-0.14 13-16 4.02-4.19 Fruit juices, pharmaceuticals 2003 282... 

---