Chromatography spray reagents

This material, normally supplied or stored in sealed ampoules and used as a paper chromatography spray reagent, is photo-sensitive. Exposure to UV light causes exothermic decomposition to acetone and chloromethane. Ampoules have burst because of pressure build-up after exposure to fluorescent or direct day light. Store cool and dark, and open ampoules with personal protection. The material also reacts violently with rubber [1,2], It should not be heated to above its boiling point [3], There is also a preparative hazard. 

Hydantoin itself can be detected ia small concentrations ia the presence of other NH-containing compounds by paper chromatography followed by detection with a mercury acetate—diphenylcarba2one spray reagent. A variety of analytical reactions has been developed for 5,5-disubstituted hydantoias, due to their medicinal iaterest. These reactions are best exemplified by reference to the assays used for 5,5-diphenylhydantoiQ (73—78), most of which are based on their cycHc ureide stmcture. Identity tests iaclude the foUowiag (/) the Zwikker reaction, consisting of the formation of a colored complex on treatment with cobalt(II) salts ia the presence of an amine (2) formation of colored copper complexes and (3) precipitation on addition of silver(I) species, due to formation of iasoluble salts at N. ... 

Patil VB, Sevaikar MT, Padalikar SV. 1987. Specific spray reagent for the detection of endosulfan by thin-layer chromatography. J Chromatogr 396 441-443. 

The consumption of the oxime can be checked by thin-layer chromatography on silica gel G with the solvent system chloro-form/methanol (95/5 v/v) and a spray reagent consisting of 5% potassium dichromate in 40% sulfuric acid. The oxime appears as an immediate dark spot and the aziridine as a yellow spot. The checkers observed identical mobilities (Rf 0.8) for both compounds. 

Early applications of thin-layer chromatography in corticosteroid analysis were mainly based on use of phenylhydrazine in sulfuric acid as a spray reagent for derivatization of the analytes (529, 530). Later applications involved use of other spray reagents including phosphoric acid in methanol, tetrazolium blue. 

Qualitative paper chromatography of the ether extractives indicated vanillin, vanillic acid, acetovanillone, and a trace of p-hydroxybenzoic acid along with many unidentified spots giving reactions with the indicator spray reagent. The ethanol extract gave most of the same spots. The water solution gave spots for oxalic acid and for several unidentified acids. 

Modification of the Activity of Alumina by Addition of Water Stationary and Mobile Phases Typical Stationary and Mobile Phase Systems Used in the Separation of Various Inorganic Ions Spray Reagents in Thin-Layer Chromatography Protocol for Reagent Preparation... 

Partridge, S.M., Aniline hydrogen phthalate as a spraying reagent for chromatography of sugars, Nature, 164, 443, 1949. 

Krebs, K.G., Heusser, D., and Wimmer, H., Spray reagents, in Thin Layer Chromatography, A Laboratory Handbook, Stahl, E., Ed., Springer-Verlag, New York, 1969, p. 854. 

Sattler, L. and Zerban, F.W., New spray reagents for paper chromatography of reducing sugars, Anal. Chem., 24, 1862, 1952 Chem. Abs., 47, 1543d, 1953. 

Spray Reagents in Thin-Layer Chromatography (continued)... 

The following section gives a summary for the preparation of the major spray reagents listed in the previous section (Spray Reagents in Thin-Layer Chromatography). Refer to the original literature for any reagents not listed here.1 4... 

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