Chromatography, selenomethionine

A method for the determination of selenomethionine in yeast by a combination of high performance liquid chromatography (HPLC) and dried droplet laser ablation ICP-MS using a species... 

Mendez, S.P., Bayon, M.M., Gonzalez, E.B., and Sanz-Medel, A. 1999. Selenomethionine chiral speciation in yeast and parenteral solutions by chiral phase capillary gas chromatography-ICP-MS. J. Anal. At. Spectrom. 14(9), 1333-1337. 

Zheng, O. and Wu, J. 1988. The determination of selenomethionine in selenium yeast by cyanogen bromide gas chromatography. Biomed. Chromatogr. 2, 258-259. 

M. B. De La Calle-Guntinas, C. Brunori, R. Scerbo, S. Chiavarini, P. Quevauviller, F. Adams, R. Morabito, Determination of selenomethionine in wheat samples comparison of gas chromatography-microwave-induced plasma atomic emission spectrometry, gas chromatography-flame photometric detection and gas chromatography-mass spectrometry, J. Anal. Atom. Spectrom., 12 (1997), 1041-1046. 

F. Yang, R. E. Sturgeon, S. McSheehy, Z. Mester, Comparison of extraction methods for quantitation of methionine and selenomethionine in yeast by species specific isotope dilution gas chromatography-mass spectrometry, J. Chromatogr. A, 1055 (2004), 177-184. 

Figure 20.2. HPLC-ICP-MS separation and quantification of selenoamino species in lobster hepatopancreas after digestion with protease type XIV (10 mg per 0.1 g sample), 0.05% m/v cysteine, pH 7.5, 37°C for 24 h. Chromatography conditions as in Figure 20.1. (a) Extract (b) extract spiked with selenocystine (c) extract spiked with selenomethionine.

Figure 20.3. HPLC-ICP-MS separation of 6 M HCl-0.05% m/v cysteine hydrolyzed selenoamino acid standards and Mugil cephalus muscle tissue, (a) Cation exchange of hydrolyzed selenomethionine and muscle tissue, Supelcosil LC-SCX cation exchange column, 20 mM pyridine-formic acid, 1.5 ml min-1, 40°C, pH 2.5. (b) Anion exchange of hydrolyzed selenocystine and muscle tissue. Chromatography conditions as in Figure 20.1.

F. Li, W. Goessler, K. J. Irgolic, Determination of trimethylselenonium iodide, selenomethionine, selenious acid and selenic acid using high performance liquid chromatography with on-line detection by inductively coupled plasma mass spectrometry or flame atomic absorption spectrometry, J. Chromatogr. A, 830 (1999), 337-344. 

M. A. Quijano, M. Gutierrez, M. C. Perez-Conde, C. Camara, Determination of sele-nocystine, selenomethionine, selenite and selenate by high-performance liquid chromatography coupled to inductively coupled plasma mass spectrometry, J. Anal. Atom. Spectrom., 11 (1996), 407-411. 

C. Devos, K. Sandra, P. Sandra, Capillary gas chromatography inductively coupled plasma mass spectrometry (CGC-ICPMS) for the enantiomeric analysis of d,l-selenomethionine in food supplements and urine, J. Pharm. Biomed. Anal, 27 (2002), 507D514. 

Do, B. et al. Application of central composite designs for optimisation of the chromatographic separation of monomethylarsonate and dimethylarsinate and of selenomethionine and selenite by ion-pair chromatography coupled with plasma mass spectrometric detection. AnaZy f 2001, 126, 594—601. 

MTs = metallothioneins NMR = nuclear magnetic resonance pKa = negative logarithm of acid dissociation constant PMT = photomultiplier tube QITMS = quadrupole ion trap mass spectrometry SBSE = stir bar sorptive extraction SDS-PAGE = sodium dodecylsulfate polyacrylamide gel electrophoresis Se-Cis = selenocystine Se-Cys = selenocysteine Se-Et = selenoethionine Se-Hcy = seleno-homocysteine Se-Met = selenomethionine SEC = supercritical fluid chromatography TBT = tributyltin TETRA = tetramethylarsonium ion Tf = transferrin TMAsO = trimethylarsineoxide TMSe = trimethylselonium ion TOFMS = time of flight mass spectrometry USN = ultrasonic nebulizer. 

Liquid chromatography/MS has also very recently been used for selenate and selenite determination and selenomethionine. And another recent liquid chromatographic hyphenated method, liquid chromatography-hydride generation atomic fluorescence spectrometry, has been used to determine selenite, selenate, selenocysteine, and selenomethionine in a single run. Reverse-phase and ion-exchange columns were used in series to accomplish the complex separation of all four of these analytes. And microchip capillary electrophoresis has most recently been used for determination of various Se-amino acids with negligible sample consumption . An excellent US Center for Disease Control summary of Se analytical methods is available. ... 

As an important example of a biochemically relevant species, Se-labeled selenomethionine can be prepared, for example, by isolation from selenized yeast, grown in a Se-labeled selenite solution. After digestion of the yeast with pro-tease/lipase solution, Se-labeled selenomethionine is isolated by size-exclusion chromatography (SEC) [72, 73]. Another important example is the synthesis of Fe-labeled transferrin [74]. The synthesis of other biologically relevant molecules, especially those of higher molecular weight, is usually too complicated, and the species-unspecific spiking mode is more convenient in these cases. 

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