Chromatography polysaccharide sulfates

Legendre, E. Etude par chromatographie d affinite hquide haute performance des interactions entre des proteines de la coagulation et des polysaccharides sulfates a activite anti-coagulante immobilises sur des supports de silice enrobee. In Thesis, University Paris, 1996 13. 

Affinity chromatography is used in the preparation of more highly purified Factor IX concentrates (53—55) as well as in the preparation of products such as antithrombin III [9000-94-6] (56,57). Heparin [9005-49-6], a sulfated polysaccharide (58), is the ligand used most commonly in these appHcations because it possesses specific binding sites for a number of plasma proteins (59,60). 

Dermatan sulfate, also termed chondroitin sulfate B, a related glycosaminoglycan constituent of connective tissue, was known to be composed of galactosamine and a uronic acid, originally believed to be glucuronic acid but then claimed to be iduronic acid based largely on color reactions and paper chromatography. However, the d or L-enantiomer status of the latter monosaccharide was not clear. Jeanloz and Stoffyn unequivocally characterized the monosaccharide as L-iduronic acid by consecutive desulfation, reduction, and hydrolysis of the polysaccharide, followed by isolation of the crystalline 2,3,4-tri-0-acetyl-l,6-anhydro-/ -L-idopyranose, which was shown to be identical to an authentic specimen synthesized from 1,2-0-isopropylidene-/ -L-idofuranose.34... 

The most common amino polysaccharide is chitosan, derived from chitin by N-deacetylation, and the N-deacetylation reaction can be conducted with little degradation of the polysaccharide chain. The depolymerization and sulfation of chitosan by sulfuric acid have been studied. Chitosan has several applications, for example, in the collection and separation of metal ions, and, in admixture with cellulose, for thin-layer, ion-exchange chromatography. - ... 

De Zoisa et al. (2008) reported the isolation and characterization of fucose-containing sulfated polysaccharide as an anticoagulant agent from the edible brown seaweed Sargassum fulvellum by means of a simple fermentation process and chromatography technique. According to their... 

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