Chromatography on an anion-exchange resin

Talvitie [31] has described a radiochemical method for the determination of plutonium in soil based on chromatography on an anion exchange resin of a 9 M hydrochloric acid extract of the sample. Following clean-up, plutonium is desorbed by reductive elution with 1.2 M hydrochloric acid, 30% hydrogen... 

Chromatography," on an Anion-exchange Resin (Dowex-1), of Certain Disaccharides ... 

B34. Boman, H. G., and Westlund, L. E., Protein chromatography on an anion-exchange resin. Arch. Biochem. Biophys. 64, 217-239 (1956). 

Tanaka, K., Ishizuka, T., and Sunahara, H., Chromatography of aliphatic amines on an anion-exchange resin, ]. Chromatogr., 172, 484, 1979. 

Thus, the choice of method depends on the application. For routine analyses of FFAs, the BDI method or modifications of it, and methods based on the Dole extraction procedure appear to be most popular (IDF, 1983), while for accurate determination of all FFAs in a product, the capillary GC (Chilliard et al., 1983 de Jong and Badings, 1990 Anderson et al., 1991) or the HPLC methods (Garcia et al., 1990) are the methods of choice. Solvent extraction using acidified ether (Salih et al., 1977) followed by separation of the free acids from fat on an anion exchange resin, [e.g., Amberlyst 26 (Needs et al., 1983)], and capillary gas chromatography of the acids or their methyl esters has been suggested by the IDF as a reference method (Anderson et al., 1991). 

If so desired, the hydrolyzed ozonation products can first be separated on an anion exchange resin into what have been described as neutral, monocarboxylic and dicarboxylic fractions (Matsumoto et al. 1986, Taneda et al. 1989). In our experience, however, acids do not always appear in the expected fraction, and there is a complicating contamination by succinic acid, apparently originating in the resin. These difficulties together with the greatly increased resolution of capillary gas chromatography (GC) have combined to render this preliminary separation unnecessary. 

K. Tanaka and K. Ohta, lon-exdusion chromatography of ethanolamines on an anion-exchange resin by elution with polyols and sugars, /. Chromatogr. A,... 

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