Thin-layer chromatography , phospholipids materials

It is assumed at this point that sufficient material and data have been accumulated, via techniques such as thin-layer chromatography, P analysis, FAE/P values, and an FAB-MS spectrum (if available), to suggest that the phospholipid is a diacylphosphatidylcholine or a closely related derivative. 

Subsequent to recovery of the total lipids of a cellular preparation as a chloroform-soluble fraction, the total phosphorus content can be determined (see Chapter 3) and then, depending on the amount of lipid phosphorus (or whether the preparation is radiolabeled or not, see below), analytical and/or preparative thin-layer chromatography can be undertaken. In either case, if the experimental protocol is centered on a signal-transduction process, then there may be insufficient material for a phosphorus analysis. In the latter instance, the cellular preparation is prelabeled with 32P or [3H]inositol and the labeled products are located by autoradiography. A preferred type of adsorbent (for thin-layer chromatography) is Merck silica gel 60 (oxalate impregnated). An effective solvent for separation of the phosphatidylinosi-tols and other lipids is chloroform-acetone-methanol-acetic acid-water (80 30 26 24 14, v/v). The approximate / values of cellular phospholipids under these conditions are presented as follows ... 

Bradova, V., Simd, F., Ledvinova, J. et al. (1990) Improved one-dimensional thin-layer chromatography for the separation of phospholipids in biological material. Journal of Chromatography, 533, 297-9. 

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