Chromatographic properties capacity factor

A variety of physical parameters have been shown to correlate with chromatographic retention. Several physical properties, measured SFC capacity factors, as well as GLC derived retention indices for the PAHs studied are listed in Table II. The capacity factors, k, were calculated from an isoconfertic-isothermal SFC separation of a mixture of the PAHs on an octadecyl bonded packed column using CC>2 as the mobile phase (4500 psi, 100°C). 

Thus (m ), the mass sensitivity of the chromatographic system depends on the detector sensitivity, column dimensions, column efficiency and the capacity factor of the eluted solute. However, irrespective of the column properties, the mass sensitivity is still directly related to the detector sensitivity. It will also be seen that the column radius will depend on the extracolumn dispersion, much of which arises from the detector connecting tubes and sensor. It follows that the design of the detector and its sensitivity has a major influence on the mass sensitivity of the overall chromatographic system. 

Martorell, C. Calpena, A.C. Escribano, E. Poblet, J.M. Freixas, J. Influence of the chromatographic capacity factor (log L) as an index of lipophilicity in the antibacterial activity of a series of 6-fluoroquinolones. Relationship between physico-chemical and structural properties and their hydrophobicity. J. Chromatogr., A 1993, 655, 177-184. 

Table 6.3 COMPARISON OF PROPERTIES AND CHROMATOGRAPHIC PERFORMANCE OF L-PheNHPh MOLECULAR IMPRINTS PREPARED UNDER DIFFERENT CONDITIONS (O SHANNESSY,1989). K D AND K L ARE CAPACITY FACTORS FOR...

For two peaks to be resolved chromatographically, the capacity ratios, Kj and K2, must be different. For analytical purposes, two interdependent chromatographic properties must be considered the chromatographic separability factor, a, and the resolution, Rs. The chromatographic separability factor is defined as... 

The support will affect the retention to an extent that depends on the properties of its surface and the components in the ion-pair [3, 43]. When silica based material is used as support, the deviations between the obtained capacity factors and those predicted from batch extraction data are usually significant [44, 45]. Careful thermostatting of the whole chromatographic system is a prerequisite for stable retention. 

In chromatography, the retention factor, or, equivalently, the capacity factor, is used to characterize the chromatographic equilibrium properties of an analyte. The retention factor of an analyte, k, is usually derived to be the product of the column phase ratio, Vr/Ve, and the distribution coefficient of the analyte between the stationary phase and eluent phase, Ca,r/ca,e, eqn [24] ... 

---