Chromatium minutissimum

The visible absorption spectrum of a partially purified enzyme of Chromatium vinosum indicates a high-spin cytochrome c (36.49). Whether cytochrome c itself can be regarded as the real enzyme or whether c is only attached to the enzyme during the purification procedure, still remains unclear. On the other hand, Ulbricht (35) could clearty demonstrate that the enzymes of Chromatium gracile and Chromatium minutissimum have no cytochrome character. 

Shuvalov, V. A., and Klimov, V. V., 1976, The primary photoreactions in the complex cytochrome P-890. P-760 (bacteriopheophytin 7gQ) of Chromatium minutissimum at low redox potentials. Biochim. Biophys. Acta, 440 587n599. 

Fig. 4. Difference absorption spectrum of photoreduced bacleriopheophytin (B

WhenphotoreducedBd> is accumulated, an EPR spectrum is also produced. For instance, in Chromatium minutissimum subchromatophores poised at -430 mV and illuminated at 293 K, Shuvalov and Klimov found the kinetics of formation of an EPR signal to be identical to that of the absorbance change due to Bd> reduction. The EPR spectrum had ag-value of 2.0025 and was 12.5 G wide. Therefore the authors attributed the EPR spectrum to a simple BO free radical as g-value and the halfwidth were consistent with that reported for the single-pigment radical in solution. 

VA Shuvaiov, W Kiimov, iN Krakhmaieva, AA Moskaienko and AA Krasnovsky (1976) Photoconversion of bacteriopheophytin in reaction centers of Rhodospiriiium rubrum and Chromatium minutissimum. Doki Akad Nauk SSSR. 227 984-987... 

In Rb. sphaeroides RCs, the primary donor in state D is re-reduced via the soluble cytochrome Cj. The structural interaction between cytochrome Cj and the RC has been studied in an X-ray diffraction experiment with co-crystals of the RC from Rb. sphaeroides and cytochrome at 4.5 A resolution (Adir et al., 1996). The structure obtained for the complex shows the cytochrome Cj interacting with the RC at the periplasmic surface of the M-subunit. Recent studies provide evidence for an RC with two cytochrome-c subunits in the purple sulfur bacterium Chromatium minutissimum (Chamorovsky et al.,... 

Bylina EJ and Wong R (1992) Analysis of spontaneous herbicide resistant revertants derived from Rhodobacter capsulatus in which Ser L223 of the reaction center is replaced with alanine. In Murata N (ed) Research in Photosynthesis, Vol 1, pp 369-372. Kluwer Academic Publishers, Dordrecht Bylina EJ, KirmeierC, McDowell L, Molten D and Yuovan DC (1988) Influence of an amino-acid residue on the optical properties and electron transfer dynamics of a photosynthetic reaction centre complex. Nature 336 182-184 Chamorovsky SK, Zakhorova NI, Remennikov SM, Sabo YA and Rubin AB (1998) The cytochrome subunit structure in the photosynthetic reaction center of Chromatium minutissimum. FEBS Lett 422 231-234... 

INTERACTION OF HYDROPHILIC SUCCINIMIDYL ETHERS WITH POLYPEPTIDES OF PIGMENT-PROTEIN COMPLEXES IN ASSEMBLY A890 FROM SULFUR PHOTOSYNTHETIC BACTERIU"M CHROMATIUM MINUTISSIMUM... 

Interaction of Hydrophilic Succinimidyl Ethers with Polypeptides of Pigment-Protein Complexes in Assembly A890 from Sulfur Photosyndietic Bacterium Chromatium Minutissimum 85... 

---