Chondroitin enzymatic polymerization

Kobayashi and coworkers also showed that hyaluronidase is able to catalyze the enzymatic polymerization of chondroitin [229], chondroitin sulfate [230], their... 

For the polysaccharide synthesis, enzymatic polymerization has been developed as a new in vitro synthesis method of natural and unnatural polysaccharides having complicated structures.The method utilizes a hydrolysis enzyme to catalyze the bond formation for the polymer construction, a reverse direction of the hydrolysis to cleave the bond. This catalysis is due to the enzymatic characteristics, where enzymes catalyze the reverse reaction involving a common intermediate in both forward and backward reactions. In nature, there are many polysaccharides having N-acetyl groups called mucopolysaccharides such as chitin, hyaluronic acid (HA), and chondroitin (Ch). 

---