Cholesterol algin

Chang YC, Chou CC (2002) Growth and production of cholesterol oxidase by alginate-immobilized cells of Rhodococcus equi No 23. Biotechnol Appl Biochem 35 69-74... 

Paxman, J. R., Richardson, J. C., Dettmar, P. W., and Corfe, B. M. (2008). Alginate reduces the increased uptake of cholesterol and glucose in overweight male subjects a pilot study. Nutr. Res. 28(8), 501-505. 

Kimura, Y., Watanabe, K., and Okuda, H. (1996). Effects of soluble sodium alginate on cholesterol excretion and glucose tolerance in rats. /. Ethnopharmacol. 54,47-54. 

TEM or SEM examination of inclusion compound...The blue stained (alginate-iodine compound) film sample and the red stained(alginate-Food color Red No.105 compound) bead sample were stained negatively with 57o uranyl acetate, made by the usual method and observed by 200kV TEM (Hitachi H-800). The shrunk and hard bead separated from acetic acid (alginate-cholesterol adduct) sample was prepared in the same manner described above(for SEM sample) and observed by SEM(Hitachi S-430). 

Cholesterol...The reduction of cholesterol in acetic acid by increasing the amount of metal alginate beads is clearly seen in Figure... 

A. Figure 6B also shows the effect of Cd-alg. or Ca-alg. on cholesterol concentration in acetic acid. The addition of metal alginate... 

Figure 6. The effect of metal alginate on cholesterol in acetic acid.

A First step beads were suspended in 30ml of acetic acid containing 5mg of cholesterol for 24hr at 30. B Metal alginate beads formed from 40mg of Na-alg. were suspended in 30ml of acetic acid containing cholesterol... 

Figure 7. Microscopic examination for inclusion compounds of alginate. A Ca-alg.-iodine film, by TEM, Bar=10nm B Ca-alg.-color(Red No.105) bead, by TEM, Bar=10nm C Ca-alg.-cholesterol bead(first step) suspended in 30ml of acetic acid containing lOmg of cholesterol, surface, fixation, Bar=lvim.

Abstract. The binding properties of granular alginic acid(H-alg.) and chitin to iodine, bromine, cadmium ion, calcium ion and cholesterol were investigated. Chitin-iodine and chitin-bromine compounds closely resemble those of H-alg. The amount of iodine included by these polysaccharides increased with a decrease in the concentration of potassium iodide (KI). The number of sugar residues bound to one iodine molecule extrapolated to 0 g of KI was around 6.0 for H-alg. and 6.4 for chitin. These saccharides, which do not form a gel in water, were found to also absorb KI and radioactive iodide or iodine in aqueous solution. H-alg. did not show as much affinity to cadmium ion and calcium ion and cholesterol in iso-propyl alcohol as metal-alginates. 

Cholesterol... None of chitin was obtained pure, so the sample of chitin obtained from carapace was positive to the cholesterol E-Test and the extinction to standard cholesterol solution increased with an increase in the amount of chitin, but any reduction of cholesterol by chitin was not observed at all. In the previous experiment the removal of cholesterol from acetic acid solution by the addition of metal alginate beads was observed. Such reduction of cholesterol from iso-propyl alcohol by the addition of metal alginate beads (unpublished), H-alg. or chitin is not observed in this experiment, so it indicates that the removal of cholesterol from the acidic solution depends on the shrinkage of the metal alginate beads, but not on the fiber chain packing. 

Category 3 Modulation of serum cholesterol level and gastrointestinal conditions Dietary fiber from psyllium husks [19] low-molecular-weight sodium alginate [9]... 

Studies have shown that orally fed alginic acid and sodium alginate depress plasma and/ or liver cholesterol levels in rats only algin with a high DP is active. Hypocholester-olemic activity was attributed to the inhibition of cholesterol absorption from the gut. 

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