Chlorpyrifos-oxon binding

H-Chlorpyrifos oxon binds covalently to rat heart M2 musearinic receptors (Bomser and Casida, 2001). The site of attachment has not been identified. When guinea pigs were treated with chlorpyrifos, diazinon, or parathion at doses too low to inhibit acetylcholinesterase activity, the M2 muscarinic receptors lost their abihty to inhibit acetylcholine release from parasympathetic nerves, causing bronchocon-striction (Lein and Fryer, 2005). 

Huff, R. A., Corcoran, J. J., Anderson, J. K and Abou-Donia, M. B. (1994). Chlorpyrifos oxon binds directly to mu.scarinic receptors and inhibits cAMP accumulatitH) in rat striatum. J. Pharmacol. Exjy They. 269, 329-335. 

Cannabinoid CB1 Chlorpyrifos oxon Binding assay Quistad et al. (2002a)... 

Grigoryan, H., Schopfer, L.M., Thompson, C.M., Terry, A.V., Masson, P., Lockridge, O. (2008). Mass spectrometry identifies covalent binding of soman, sarin, chlorpyrifos oxon, diisopropyl fluorophosphate, and FP-biotin to tyrosines on tubulin a potential mechanism of long term toxicity by organophos-phoms agents. Chem. Biol. Interact. April 22. (Epub ahead of print)... 

Katz, E.J., Cortes, V.I., Eldefi awi, M.E., Eldefi awi, A.T. (1997). Chlorpyrifos, parathion, and their oxons bind to and desensitize a nicotinic acetylcholine receptor relevance to their toxicities. Toxicol. Appl. Pharmacol. 146 227-36. 

Kousba, A. A., Sultatos, L. G.. Poet, T. S., and Timctuilk, C. (2004). ComparLson of chlorpyrifos-oxon and paraoxon acetylcholinesterase inhibition dynamics Potential role of a peripheral binding site. Toxicol Sci. 80,239-248,... 

T. S. Poet, C. Timchalk, Comparison of Chlorpyrifos-Oxon and Paraoxon Acetylcholinesterase Inhibition Dynamics Potential Role of a Peripheral Binding Site, Toxicd. Sci., 2004, 80, 239-248. 

Howard MD, Pope CN. In vitro effects of chlorpyrifos, parathion, methyl parathion and their oxons on cardiac muscarinic receptor binding in neonatal and adult rats. Toxicology 2002 170(1—2) 1—10. 

Beianeoun, A. M., and Carr, R. L, (2004). The effect of chlorpyrifos and chlorpyrifo,s-oxon on brain cholinesterase, muscarinic receptor binding, and neurotrophin levels in rats following early postnatal exptisure. Toxicol. Sci. 77,63-71. 

Cultures treated 1 hr with chlorpyrifos, chlotpyrifos-oxon, or crichloropyridinol in medium with (astrocytes) or without serum (neurons) cortical neuron.s also treated with trichloropyridlnol 7 day.s viability. AChF. activity, and immunobloiting for phosphorylated Ca- /cAMP response element binding protein (pCREB) measured. 

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