Chlorophylls chlorophyll-binding proteins

U. Johanningmeier, S. H. Howell (1984) Regulation of light-harvesting chlorophyll-binding protein mRNA accumulation in Chlamydomonas reinhardtii. Possible involvement of chlorophyll synthesis precursors. J. Biol. Chem., 259 13541-13549... 

Genes down-Rice regulated by low temperature Rubisco ssu Cab Chlorophyll binding protein Hahn Walbot, 1989... 

S Jansson, E Pichersky, R Bassi, BR Green, M Ikeuchi, A Melis, DJ Simpson, M Spangfort, LA Staehelin and JP Thornber (1992) A nomenclature for the genes encoding the chlorophyll alb-binding proteins of higher plants. Plant Mol Biol Report 10 242-253... 

Haxo FT, Kycia, JH, Somers GF, Bennet A and Siegelman HW (1976) Peridinin-chlorophyll a-binding protein of the dinoflagellate Amphidinium carterae (Plymouth 450). Plant Physiol 57 297-303... 

Norris BJ and MUler DJ (1994) Nucleotide sequence of a cDNA clone encoding the precursor of the peridinm-chlorophyll a-binding protein from the dinoflageUate Symbiodinium sp. Plant Mol Biol 24 673-677... 

Jahns P (1995) The xanthophyll cycle in intermittent light-grown pea plants Possible functions of chlorophyll a/ -binding proteins. Plant Physiol 108 149-156 Jahns P and Miehe B (1996) Kinetic correlation ofrecovery from photoinhibition and zeaxanthin epoxidation. Planta 198 202-210... 

Morgan TR, Shand JA, Clarke SM et al. Specific requirements for cytochrome c-550 and the manganese- stabilizii protein in photoautotrophic strains of Synechocystis sp. PCC 6803 with mutations in the domain Gly-351 to Thr-436 of the chlorophyll-binding protein CP47 Biochemistry 1998 37 14437-14449. 

Barber J, Morris EP, Buchel C. Revealing the struemre of the photosystem two chlorophyll binding proteins, CP43 and CP47. Biochim Biophys Acta 2000 145 239-247. 

Mori H, Yamamoto Y. Deletion of antenna chlorophyll-a-binding proteins CP43 and CP47 by Tris-treatment of PSII membranes in weak l t Evidence for a photod adative effect on the PSII components other than the reaction center-binding proteins. Biochim Biophys Acta 1992 100 293-298. 

Mori H, Yamashita Y, Akasaka T et al. Further characterization of the loss of antenna chlorophyll-binding protein CP43 from photosystem II during donor-side photoinhibidon. Biochim Biophys Acta 1995 1228 37-42. 

Liu, H., Huang, R.Y.C., Chen, J., et al. (2011) Psb27, A Transiently Associated Protein, Binds to the Chlorophyll Binding Protein CP43 in Photosystem II Assembly Intermediates. Proceedings of the National Academy of Sciences of the United States of America, 108 (45), 18536-18541. 

II. It is likely to be located near the reaction center Di/D2/cyt6-559 complex (6, 7, 8) but other possibilities are near the chlorophyll a binding proteins Cp47 (9,10) and Cp43 (11) subunits. 

Fractionation of PSII membranes into discrete subsets. The methods of lEF and immunoblotting give us the possibility to separate and identifie chlorophyll-binding proteins both in native conditions, usefull for the study of protein—protein interactions and fully denaturing conditions indispensable when the quantitative relations between apoproteins must be determined. These... 

Shown in this model are phycobilisome (PBS), reaction center of PSII (RCII), PSn core chlorophyll-binding proteins (CP), and oxygen-evolving complex (OEC). Darkened stmctures indicate components in a nonfunctional form. 

I.the pigment-protein complex PSl of cyanobacteria is a compact closed body composed of 5 low-molecular polypeptides surrounded by two chlorophyll-binding proteins (the pigment antenna system) forming a protective shield which hinders access to small polypeptides from the surface of the complex or from the surface of the thylakoid membrane. 

These results were confirmed by immuneblot assay of both thylakoids and CPI. The level of LHCI in CPI from chlorina- grown at 15 C was less than 5% of that in wild type, and returned to normal levels after 5 days at 22 C (Figure 2). In contrast, the chlorophyll alb-binding protein CP29 was present in near normal levels in thylakoids, and was absent from the CPI and PSI-200 preparations. 

It is possible that the 31 kD polypeptide shares some homology with an intrinsic, chlorophyll binding protein of similar MW observed in Prochloron and Cyanobacteria (5, 6). Details of the isolation and characteristics of this protein will be reported elsewhere. 

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