Chitin bead

Fig. 21 Chitin binding of 6xHis-tagged resilin with chitin-binding domain (6 H-resChBD) as compared to 6xHis-tagged resilin without chitin-binding domain (6 H-res). T total protein after affinity chromatography purification, B bound protein eluted from chitin beads, UB unbound protein. Reproduced from [187] with permission from The American Chemical Society, copyright 2009...

Fig. 3. (Continued) cysteine-possessing protein. The protein of interest (protein 2) is expressed as CBD-intein-protein 2 precursor and purified by the chitin beads. Temperature- or pH-induced intein cleavage results in proteins with an N-terminal cysteine residue. Finally, the EPL (dotted line) of the protein thioester and the cysteine containing protein, which can be also obtained synthetically, proceeds under NCL conditions.

Zhou D, Zhang LN, Zhou JP, Guo SL (2004) Cellulose/chitin beads for adsorption of heavy metals in aqueous solution. Water Res 38 2643-2650... 

Zhou D, Zhang L, Guo SL (2005) Mechanisms of lead biosorption on cellulose/chitin beads. Water Res 39 3755-3762... 

Prepare two chitin column for affinity-chromatographic purification of expressed fusion protein. Therefore, fill slurry of chitin beads in a column and allow them to decant (final bed volume 15 mL). Wash chitin beads with water (20 bed volumes) and equilibrate with column buffer containing 3 M urea (10 bed volumes). 

If you do not own a French press, apply sonication or break down cells by repeating freeze-and-thaw cycles. Avoid lysozyme for cell lysis, because it will damage and drastically reduce the life-span of your chitin beads ... 

Various forms of chitin-based products are available for medical applications, such as finely divided powder, nonwoven fabrics, porous beads, lyophilized soft fleeces or gels, gauges, laminated sheets, and transparent films. Water-soluble derivatives of chitin in isotonic saline can be administered intramuscularly or intravenously. Certainly, there is considerable biochemical evidence linking NAG with the metabolism of the hexamines, which are assumed to originate and cross-link wound collagen. 

Fibres were first developed by Austin [60] and then by Hirano [61-63] in solvents mentioned previously, especially the DMAc/LiCl system. The fibres were obtained by wet-spinning [63]. A recent review presents the different fibres obtained from chitin solution and some of their physical properties [27]. In addition, chitin solutions may be casted to obtain films [64,65] or regenerated under sponge or bead conformation in dependence of the use. Fibres were often proposed for textile applications [66-68]. 

Elevan, Y. and Murat, B. (2003) Preparation and cheuacterization of physiceil gels and beads from chitin... 

As chitosan is versatile, it can be manufactured into films, membranes, fibers, sponges, gels, beads, and nanoparticles, or supported on inert materials. The utilization of these materials presents many advantages in terms of applicability to a wide variety of process configurations. The various forms of chitin and chitosan are discussed below. 

---