Chiral method development screening conditions

Reversed phase chiral separations are desired simply for efficiency in generating results from laboratories whose instrumentation is routinely configured to run in reversed and not normal phase modes.Normal phase conditions are less attractive to the analytical chemist for this reason and deter laboratory efficiency. Typical commercial chiral LC columns found on pharmaceutical reversed phase LC chiral method development screens are listed in Table 8. Table 11 shows suggested chromatographic conditions employed in reversed phase chiral screening. 

In this way, we aim to give an overview of what can be used as a separation technique and which conditions will most likely give an (beginning of) enantiomer separation after a first screening. Chiral method development starter kits are also available and evaluated in some papers [2], but we will not focus on this kind of applications. 

Method development remains the most challenging aspect of chiral chromatographic analysis, and the need for rapid method development is particularly acute in the pharmaceutical industry. To complicate matters, even structurally similar compounds may not be resolved under the same chromatographic conditions, or even on the same CSP. Rapid column equilibration in SFC speeds the column screening process, and automated systems accommodating multiple CSPs and modifiers now permit unattended method optimization in SFC [36]. Because more compounds are likely to be resolved with a single set of parameters in SFC than in LC, the analyst stands a greater chance of success on the first try in SFC [37]. The increased resolution obtained in SFC may also reduce the number of columns that must be evaluated to achieve the desired separation. 

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