Chip single channel

Reactor 15 [R 15] Single-channel Chip Micro Reactor... 

Reactor type Single-channel chip micro reactor Zeolite catalyst layer thickness 3 pm... 

Figure 5.11 Schematic of the single-channel chip micro reactor. Divergent inlet channels (A) secondary inlet channel (B) serpentine irradiation channel (center) outlet channel (Q [21. ...

Using a single-channel chip micro reactor, singlet oxygen is generated by photochemical means in presence of catalytic amounts of Rose Bengal [21]. By [4 -i- 2] cycloaddition of this oxygen species to a-terpinene, the product ascaridole is obtained. 

Chip Design Flow MS Mode Analytes Single-Channel ESI Chip Sample Treatment Separation Ref. 

Figure 14 (a) Channel layout of a single-channel chip, (b) Layout of channels for... 

Figure 14 (A) Schematic of a chip for flow-through PCR. Three well-defined zones are kept at 95, 77, and 60°C by means of thermostated copper blocks. The sample is hydrostatically pumped through a single channel etched into the glass chip. The channel passing through the three temperature zones defines the thermal cycling process. (B) Layout of the device used in the study. The device has three inlets on one side of the device and an outlet on the opposite side. Only two inlets were used, one carrying the sample, the other bringing a constant buffer flow. The chip incorporates 20 identical cycles, except for the first one, which includes a threefold increase in DNA melting time. (Reprinted with permission from Ref. 98.)...

The PCR channel for these CFPCR devices can also consist of either capillary tubes [99, 100] or an on-chip annular channel [95, 101]. The serpentine channel formats on a monolithic chip can utilize thermal insulation with the aid of air gaps [102, 103] or by utilizing glass chips with a low thermal conductivity [104, 105]. Recently, a novel spiral chaimel configuration was also used to perform CFPCR on a single PC wafer with a circular arrangement of three temperature zones, allowing for a compact footprint and a minimal number of heaters for temperature control [106, 107]. CFPCR microfluidics can also use a uiudirectional PCR or oscillatory flow [108]. 

For mechanical lysis, nanostructured filter-Uke contractions are employed in microfluidic channels with pressure-driven cell flow. Prinz et al. utilized rapid diffusive mixing to lyse Escherichia coli cells and trap the released chromosome via dielectrophoresis (DEP). Kim et al. developed a microfluidic compact disk platform for mechanical lysis of cells using spherical particles with an efficiency of approximately 65 % however, this method is difficult to be apphed for single-cell analysis. Lee et al. fabricated nanoscale barbs in a microfluidic chip for mechanical cell lysis by shear and frictional forces. Munce et al. reported a device to lyse individual cells by electromechanical shear force at the entrance of 10 mm separation channels. The contents of individual cells were simultaneously injected into parallel channels for electrophoretic separation, which can be recorded by laser-induced fluorescence OLIF) of the labeled cellular contents. The use of individual separation channels for each cell separation eliminated possible cross-contamination from multiple cell separations in a single channel. 

This differential RPS chip showed comparable sensitivity to commercial flow cytometers with simple, cheap, and compact system on a micro glass slide. Drawbacks of the method are the baseline drifting by multiple-stage differential amplification and the low throughput of using single-channel detection. 

Figure 8.23 Micro-channel reactor configuration. W, width of total channels 44.5 mm Wj, width of single channel 1.0 mm Wf, width of fin 0.5 mm h, depth of channel 1.25 mm H, thickness of chip 2mm L, length of channel 30mm.

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