Chemiluminescence nitrogen detection

Harrison, C.R., Lucy, C.A. (2002). Determination of zwitterionic and cationic surfactants by high-performance liquid chromatography with chemiluminescent nitrogen detection. J. Chromatogr. A 956(1-2), 237-244. 

Relative purity measurement and the relative purity-based reaction optimization have long been used in combinatorial synthesis. In order to make high-through-put purification a success, the yield-based optimization is essential. Chemiluminescent nitrogen detection (CLND) [4] with HPLC determines the quantitative yield after each reaction step during the library feasibility and rehearsal stages. The yield of each synthetic step provides guidance for the final library synthesis. 

Gradient RP-HPLC separation with 0.1% formic acid and methanol as mobile phase components (0.25 mL/min) combined either with ESI-time-of-flight [23] MS detection or with chemiluminescence nitrogen detection (CLND) was used to identify and, respectively, quantify illicit drugs in seized material without primary reference standards [24], The method exploits the accurate mass measurement provided by TOE MS, enabling the unequivocal identification of molecular formula of the unknown analyte, and the CNLD equimolar response to nitrogen. 

In LC/MS analysis of combinatorial libraries, the MS determines the product identity and its purity is determined by other on-line detection techniques such as UV, evaporative light scattering detection (ELSD), and chemiluminescent nitrogen detection (CLND).17-20 UV detection is used here to assess product purity based on the assumption of similar absorption coefficients at 214 nm for the desired product and the side-products. 

Fitch WL, Szardenings AK, Fujinari EM. Chemiluminescent nitrogen detection for HPLC an important new tool in organic analytical chemistry. Tetrahedron Lett 1997 38 1689-1692. 

Bizanek R, Manes JD, Fujinari E, Chemiluminescent nitrogen detection as a new technique for purity assessment of synthetic peptides separated by reversed-phase HPLC, Peptide. Res., 9(l) 40 44, 1996. 

The physical quality of samples is assessed via analytical methods. Liquid chromatography with mass spectrometry (LC-MS) is the most common instrumentation used to determine sample presence and purity (Kerns et al., 2005). Chemiluminescent nitrogen detection (CLND), charged aerosol detection (CAD) and other techniques have also been used to determine concentration (Popa-Burke et al., 2004 Gamache et al., 2003). 

Many other on-line detectors suitable for SEC columns as reviewed [154], including chemiluminescent nitrogen detection, dynamic surface tension detection, high frequency detection and Fourier transform infrared detection, can be applied to FFF the latter being capable of delivering polymer compositions online. 

Searle, P Hochlowski, J. High Throughput Purification of Parallel Synthesis Samples An Integrated Preparative-LC/MS System with Quantitation by Chemiluminescent Nitrogen Detection, presented at The HPLC 2002, Montreal, Canada, June, 2002. 

Fitch, W.L. Szardenings, A.K. Fujinari, E.M. Chemiluminescent Nitrogen Detection for HPLC An Important New Tool in Organic Analytical Chemistry, Tetrahedron Lett. 38, 1689-1692 (1997). 

Pungent Flavor Profiles and Components of Spices by Chromatography and Chemiluminescent Nitrogen Detection... 

Sulfate fertility, role in flavor intensity of onions, 46/,47-49 Supcreritical carbon dioxide, extraction of Allium species, 113-123 Supercritical fluid chromaiography-chemiluminescent nitrogen detection, 102,104/... 

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