Chemicals mutagenicity screening

Rinkus SJ, Legator MS. 1980. The need for both in vitro and in vivo systems in mutagenicity screening. In de Serres FJ, Hollaender A, eds. Chemical mutagens Principles and methods for their detection. New York, NY Plenum Press, 365-473. 

Kada, T., Hirano, K. Shirasu, Y. (1980) Screening of environmental chemical mutagens by the... 

The liquid feed and products were screened for chemical mutagens by use of the Ames assay (1, 4). 

Kada, T., K. Hirano, and Y. Shirasu. Screening of environmental chemical mutagens by the Rec-assay system with Bacillus subtilis, pp. 149-173. In F.J. de Serres and A. Hollaender, Eds. Chemical Mutagens Principles and Methods for Their Detection. Vol. 6. 

Other microbial mutation, repair and recombination assays have also been used in chemical screening, however, the data base for carcinogenicity-mutagenicity correlation established with these in vitro test systems is relatively limited at this time.62-70 At present, the Ames Salmonella assay appears to be the most generally applicable screen for the detection of chemical mutagens. No other mutagenesis assay has been shown to respond to such a wide group of chemical types as the Salmonella mutants. 

Kanematsu, N., Hara, M., and Kada, T., REC assay and mutagenicity studies on metal compounds screening of environmental chemical mutagens by the REC-assay system with Bacillus subtilis. Mutat. Res., 77, 109-116, 1980. 

DETECTION OF MUTAGENIC SUBSTANCES AND APPLICATION OF MUTAGEN SCREENING TESTS FOR THE DETECTION OF CHEMICAL CARCINOGENS... 

Application of Mutagen Screening Tests for the Detection of Chemical Carcinogens... 

Kada, T., Tutikana, K., and Sadale, Y., "In vitro and Host-Mediated Rec-assay Procedures for Screening Chemical Mutagens and Phloxine, a Mutagenic Red Dye Detected", Mutat. Res. (1972), 16 165-174. 

The basic minimum data base, which might be considered as acceptable would include acute oral and dermal LDj- s, acute inhalation LC eye, and skin irritation studiesr reeding studies of at least 90 days duration on adequate numbers of animals of at least two species, one of which would be a non-rodent species, absorption, distribution and excretion data in one of the species used in the 90 day studies, mutagenicity screening studies, possibly a multigeneration reproduction study, and any special studies indicated by the chemical structure of the compound under test. 

The compound had no effect on the microbial model organisms tested so an in-depth chemical genetic screen for resistant Arabidopsis mutants was employed. Seven resistant mutants exhibiting 5- to 120-fold resistance to ATA7 relative to wild type were obtained from a screen of420,000 EMS-mutagenized M2 seedlings... 

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