Characterization of Glycopeptides

Rohrer, J. S., Cooper, G. A., and Townsend, R. R., Identification, quantification, and characterization of glycopeptides in reversed-phase HPLC separations of glycoprotein proteolytic digests, Anal. Biodiem., 212, 7, 1993. 

Townsend RR, Basa LJ, Spellman MW (1996) Identification and characterization of glycopeptides in tryptic maps by high-pH anion exchange chromatography. In Karger BL,Hancock (eds) Methods enzymol,vol 271. Academic Press, NY, p 135... 

For novel oligosaccharides for which H-n.m.r. data have not previously been documented in the literature, it is necessary to supplement the H-n.m.r. data with composition, sequence, and linkage information provided by chromatographic and m.s. analyses. An illustrative example is the characterization of the following oligosaccharide of the glycopeptides of fetal gastrointestinal mucins of meconium (74) ... 

The development glycopeptide libraries obtained by the split-mix method is severely hampered by the lack of concurrent development of a general, facile separation and characterization technology. Some headway has been made with chemical coding of the libraries, but very few direct methods of analysis exist. One promising method that could be applied to the direct characterization of both types of libraries is mass spectrometry. More specifically, post-source-decay matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (PSD-MALDI-TOF-MS) and CID-FAB/MS/MS have been used to characterize glycopeptides.53-55... 

Arthur, M. Molinas, C. Depardieu, E Courvalin, P Characterization of Tnl546, a Tn3-related transposon conferring glycopeptide resistance by synthesis of depsipeptide peptidoglycan precursors in Enterococcus faecium BM4147. J. Bacteriol., 175, 117-127 (1993)... 

K. Alving, H. Paulsen, and J. Peter-Katalinic, Characterization of O-glycosylation sites in MUC2 glycopeptides by nanoelectrospray QTOF mass spectrometry, J. Mass Spectrom., 34 (1999) 395-407. 

Li TL, Huang F, Haydock SF et al (2004) Biosynthetic gene cluster of the glycopeptide antibiotic teicoplanin characterization of two glycosyltransferases and the key acyltransfer-ase. Chem Biol 11 107-119... 

Clark, R.S., Banerjee, S., and Coward, J.K. (1990) Yeast oligosaccharyltrans-ferase glycosylation of peptide substrates and chemical characterization of the glycopeptide product./. Org. Chem. 55, 6275-6285. 

Z. Darula and K. F. Medzihradszky, Affinity enrichment and characterization of mucin core-1 type glycopeptides from bovine serum, Mol. Cell. Proteomics, 8 (2009) 2515-2526. 

Glycopeptide resistance in a cluster of three clinical isolates of vancomycin-resistant Enterococcus faecium was due to vanD, which was located on the chromosome and was not transferable to other enterococci These isolates were indistinguishable but differed from the strain in which vanD-mediated resistance has been reported previously (102). A type of acquired glycopeptide resistance, named vanE, has been characterized in E. faecalis BM 4405. It results in low-level resistance of vancomycin but susceptibility to teico-planin (103). Defects in penicillin-binding protein 4 result in a distorted peptidoglycan composition of the cell of vancomycin-resistant and teicoplanin-resistant laboratory mutants of S. aureus and are suggested to be part of the mechanism of glycopeptide resistance in these microbes (104). 

The first structural work on vancomycin were degradation studies carried out by Marshall [208] followed by extensive NMR examinations in the laboratory of Williams [209]. On the basis of these pioneering studies and on the X-ray analysis of the degradation product CDP-1 [210], Harris and Harris published the complete structure of vancomycin in 1982 [211]. The determination of the vancomycin structure then served as the basis for the structural characterization of hundreds of natural and semisynthetic glycopeptide antibiotics. 

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