Cerenkov

Carbon, analysis, 217, 318, 319 Carbon brushes, examination by x-ray absorptiometry, 97 Cathode follower, 60 Cauchois arrangement, 119, 120, 123 Caustic circle, 119, 120 Cells for liquids, 191, 194 Cements, analysis in Applied Research Laboratories PXQ, 260, 261 Ceramics, analysis by x-ray emission spectrography, 222-224 Cerenkov radiation, 43 Cesium, determination by x-ray emission spectrography, 328 Characteristic-line generator of Eng-strom, 144... 

The immobilized immunoprecipitates are washed twice with lysis buffer containing 0.5 MNaCl and twice with buffer A. The beads are resuspended in 20 /il of kinase buffer also containing the appropriate concentration of the specific peptide. Reactions should also be set up without peptide as a negative control for nonspecific or self-incorporation of radiolabel. To start the reactions, 5 /il of ATP is added (final concentration 0.1 mM unlabeled ATP, 1 /iCi [7 -32P]ATP (per assay) in kinase buffer). The assays are allowed to proceed for 15 to 30 min at 30° with constant shaking at 900 rpm, and stopped by spotting 20 /il of the sample (slurry) onto a square (1.5 X 1.5 cm) of phosphocellulose (P81) paper. The P81 papers are immediately immersed in 500 ml of 1% (v/v) orthophosphoric acid, and then washed 3 times with the same solution (to remove the excess ATP). The washes therefore contain almost all of the radiolabel and must be handled carefully and disposed of appropriately. The papers are briefly rinsed in ethanol and air-dried. The incorporation of 32P-label is measured by Cerenkov counting. 

Figure 11.4 Analysis of in vitro synthesized RNAs. 32P-Radiolabeled RNAs (48 nucleotides) capped with m7Gp3G (A and C) or m27,3 °Gp3G (B and D) were digested with either RNase T2 (A and C) or RNase T2 plus tobacco acid pyrophosphatase (TAP) (B and D) followed by anion-exchange HPLC on a Partisil 10SAX/25 column as described in the text. Fractions of 1 ml were collected, and the Cerenkov radiation was determined. The elution times of the following standard compounds, detected by ultraviolet (UV) absorption, are indicated with arrows 3,-CMP (Cp), S UMP (Up), 37-AMP (Ap), 3 -GMP (Gp), 3, 5 -m7GDP (pm7Gp), 3, 5 -GDP (pGp), 5 -GDP (p2G), 5 -GTP (p3G), and guanosine-SCtetraphosphate (P4G).

Cerenkov radiation accounts for a very minor part of the energy loss of fast electrons. Its main importance is for monitoring purposes and establishment of a reference time, since it is produced almost instantaneously with the passage of the particle. Katsumura et al. (1985) have observed a very fast rise of solute fluorescence attributable to the Cerenkov effect the G value for this process is estimated to be -0.02. 

Jelly, J.V. (1958), Cerenkov Radiation and Its Application, Pergamon, Elmsford, NY. 

Using the refractive index value of the pyrazine LB film, we calculated the mode dispersion curves of the TM fundamental and the TM second-harmonic waves in the waveguide device composed of a waveguiding pyrazine layer and a fused quartz substrate when Nd YAG laser is used as a fundamental light (Fig. 18). These curves show that the Cerenkov type phase matching is possible in the range of the thickness from 410 nm to 510 nm. 

Cerenkov radiation emission of blue hght by particles moving faster than light in a medinm other than the vacuum (note that in a medinm with refrachve index n, hght moves with speed v = cjn)... 

The examples for radioactive labeling by phosphorus-32 ( P) and iodine-125 ( 1) in this chapter were chosen for two reasons on one hand, they are relatively easy to do, and on the other hand, the measurement of radioactivity is simple. is counted in water in a liquid scintillation counter by measuring the Cerenkov radiation and is measured in a gamma counter. Both isotopes may be detected also by autoradiography. A further advantage of both isotopes is their short half-life, which eases the disposal of nuclear waste. 

The scintillation cocktail chosen depends on the radioisotope and the type and amount of solvent containing the labeled compound. High-energy (3 emitting isotopes, such as 32-phophorus, are detectable by several methods such as liquid scintillation (Cerenkov... 

I Twin Linac, Cerenkov, Absorption, Univ, Tokyo (1985) ... 

The jitter between the laser pulse and the electron pulse was estimated from the measurement using a streak camera (C1370, Hamamatsu Photonics Co. Ltd.), because the jitter is one of important factors that decide the time resolution of the pulse radiolysis. The jitter was several picoseconds. To avoid effects of the jitter on the time resolution, a jitter compensation system was designed [74]. The time interval between the electron pulse (Cerenkov light) and the laser pulse was measured by the streak camera at every shot. The Cerenkov radiation was induced by the electron pulse in air at the end of the beam line. The laser pulse was separated from the analyzing light by a half mirror. The precious time interval could be... 

Jonah et al. [178] found that the fluorescence emitted by 9,10-diphenyl-anthracene (or by p-terphenyl) in cyclohexane solution after photostimulation with Cerenkov radiation both grew-in and decayed more rapidly than when the solution was pulse-radiolysed (see Fig. 34). The delay in the formation of the aromatic excited state ( 0.5 ns) was attributed to the slowness of charge capture and of diffusion together of ions to form an excited state (Sect. 3.4). 

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