Cerebellar cortex zebrin

Eisenman EM, Hawkes R (1993) Antigenic compartmentation in the mouse cerebellar cortex Zebrin and HNK-1 reveal a complex, overlapping molecular topography. J. Comp. Neurol, 335, 586-605. 

Leclerc N, Dore L, Parent A, Hawkes R (1990) The compartmentalization of the monkey and rat cerebellar cortex zebrin I and cytochrome oxidase. Brain Res., 506, 70-78. 

Parasagittal zonation in the cerebellar cortex Antigenic compartmentalization for Zebrin and other markers... 

The distribution of 5 -nucleotidase (5 -N) (Section 3.5.) in alternate longitudinal bands of high and low enzyme activity in the molecular layer of the cerebellar cortex of the mouse (Scott, 1963,1964,1965,1967) was the first evidence for the biochemical compart-mentalization of the cerebellar cortex. The pattern of 5 -N-positive and -negative zones is complete in the sense that it is present in all the lobules of vermis and hemisphere and unequivocal, because, in the mouse at least, the bands are clearly delineated (Marani, 1986). The 5 -N band pattern is very similar, if not identical, to the more recently described distribution of Purkinje cells in the rat, reacting with Purkinje cell-specific monoclonal antibodies to Zebrin-I (mabQl 13) (Eisenman and Hawkes, 1989). 

Fig. 136. The reconstruction of parasagittal bands of Zebrin I (mabQl 13-immunoreactive) Purkinje cells in the adult rat cerebellar cortex as seen from the anterior (a) and posterior (b). The band pattern is based upon the serial reconstruction of nine complete and five partial cerebella from sections cut in the horizontal plane and four complete reconstructions from sections cut coronally. Bands PI + through P7+ are labelled. Hawkes and Leclerc (1987).

Zebrin-immunoreactive and non-immunoreactive Purkinje cells are distributed in parallel longitudinal bands in the cortex of rat cerebellum (Hawkes et al., 1985) and distribute their axons to different parts of the cerebellar nuclei (Hawkes and Leclerc, 1986). Light microscopical observations showed that all Zebrin positive boutons on the soma and dendrites of large central nuclear cells contained GAD, and that most GAD-positive boutons on individual cells either were Zebrin-positive or -negative. The two populations of Purkinje cells, therefore, terminate on different central nuclear cells. Zebrin-positive Purkinje cells of the vermis projected to the caudal part and a majority of Zebrin-negative Purkinje cells to the rostral part of the fastigial nucleus of the rat. 

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