Ceramides neutral glycosphingolipid

Domon, B., Vath, J. E. and Costello, C. E. Analysis of derivatized ceramides and neutral glycosphingolipids by high-performance tandem mass spectrometry. Anal Biochem, 184, 151,1990. 

Ann and Adams (1993) have explored the use of alkali metal ions as adducts to improve structural determination of other types of sphingolipids. CID spectra of [M + Cat]" ions (Caf = alkali metal ions) of ceramides and neutral glycosphingolipids provide immediate information about the lengths of the sphingolipid base and A -acyl chains and, most importantly, the locations of substituents on the A -acyl chain. 

Ann Q, Adams J. Structure determination of ceramides and neutral glycosphingolipids by collisional activation of [M +Li] ions. J Am Soc Mass Spectrom. 1992 3 260-3. 

The five major neutral glycosphingolipids of human plasma have been characterized and each has a structure which has been identified previously. One of these glycolipids, D-glucosylceramide, and possibly a second, lactosyl ceramide, exchange between plasma and erythrocyte pools. The conclusion is drawn in terms of the relative roles of carbohydrate and lipid moieties of the glycosphingolipids in maintaining that association with erythrocyte membranes. 

A sulphated neolactotetraosylceramide isolated from hog gastric mucosa was characterized as (19) by conventional procedures. A unique D-gluco-sphingolipid pattern which has not been previously encountered in any type of tissue or cell has been seen in chicken egg-yolk. The structures were determined and the major ones were found to be NeupiVAc -> o-Galp - ceramide, NeupAAc lactosyl ceramide, and NeupAAc NeupAAc -> lactosyl -> ceramide. The only other neutral glycosphingolipid was D-galactosylceramide. 

Takamatsu, K., Mikami, M., Kiguchi, K., Nozawa, S. Iwamori, M. Structural characteristics of the ceramides of neutral glycosphingolipids m the human female genital tract—their menstrual cycle-associated change in the cervical epithelium and utenne endometrium, and their dissociation in the mucosa of the fallopian tube with the menstrual cycle. Biochim. Biophys. Acta 1165, 177-182 (1992). 

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