Countercurrent chromatography centrifugal partition chromatograph

Countercurrent Chromatography Countercurrent chromatography (CCC) or centrifugal partition chromatography (CPC) has gained much attention in recent years for isolation of polyunsaturated fatty acids. This new liquid chromatographic technique uses liquid-liquid partition, countercurrent distribution of solute mixmre between two liquid phases, in the absence of a solid support, to perform separation of complex mixture of chemical substances (44, 45). [Pg.1946]

High-speed coxmtercurrent chromatography (HSCCC) and centrifugal partition chromatography (CPC) refer to particular types of hydrodynamic and hydrostatic sohd support-free chromatographic systems, respectively. Countercurrent chromatography was firstly introduced by Ito, Harada, and Aoki in 1966 [6], while... [Pg.2147]

High-speed countercurrent chromatography (HSCCC) is a relatively new technique. It is a liquid-liquid partitioning chromatographic method in which the stationary phase is immobilized by a centrifugal force. HSCCC is the most advanced form of CCC in terms of partition efficiency and separation time. As solvent system, a mixture in different ratio is usually used, depending on the flavonoids nature ethyl acetate/... [Pg.176]