Centrifugal partition chromatographic techniques

El Tayar, N. Tsai, R.-S. Vallat, P. Altomare, C. Testa, B., Measurement of partition coefficients by various centrifugal partition chromatographic techniques. A comparative evaluation, J. Chromatogr. 556, 181-194 (1991). 

El Tayar, N., R.-S. Tsai, P. Vallat, C. Altomare, and B. Testa. 1991. Measurement of Partition Coefficients by Various Centrifuge Partition Chromatographic Techniques A Comparative Evaluation. /. Chromatog. 556 181-194. 

To sum up, a lot of experience is needed to determine log P values by the classical shake flask method. Alternatives have been developed and compared with each other, e.g. filter probe methods [219, 220], the AKUFVE method [221], and different centrifugal partition chromatographic techniques (which correspond to true partitioning because only two immiscible liquid phases and no solid support are involved) [222-225]. As the scope and limitations of most of these techniques have been reviewed [173, 217, 218, 225], they shall not be discussed here in detail. 

Countercurrent Chromatography Countercurrent chromatography (CCC) or centrifugal partition chromatography (CPC) has gained much attention in recent years for isolation of polyunsaturated fatty acids. This new liquid chromatographic technique uses liquid-liquid partition, countercurrent distribution of solute mixmre between two liquid phases, in the absence of a solid support, to perform separation of complex mixture of chemical substances (44, 45). 

High-speed countercurrent chromatography (HSCCC) and centrifugal partition chromatography (CPC) are two very promising chromatographic techniques which have been successfully applied to the purification of natural products. 

High-speed countercurrent chromatography (HSCCC) is a relatively new technique. It is a liquid-liquid partitioning chromatographic method in which the stationary phase is immobilized by a centrifugal force. HSCCC is the most advanced form of CCC in terms of partition efficiency and separation time. As solvent system, a mixture in different ratio is usually used, depending on the flavonoids nature ethyl acetate/... 

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