Centrifugal layer chromatography

CENTRIFUGAL LAYER CHROMATOGRAPHY. Analytical or preparative chromatography in which solvent is driven through the layer by centrifugal force in circular, anticircular, or linear... 

Cations, detection and identification of, 519-520 Cellulose, 108-109,925 detection of peptides and proteins on, 435-437 enantiomeric separations on triacetylcellulose thin-layer plates, 630-631 for separation of carbohydrates, 485-487 Centrifugal layer chromatography (CLC), 79,172 Cephalosporins, 452-457 Chemically modified sorbents, 110-119 hydrophillic modified phases, 110-112 hydrophillic modified precoated layers, 113-1 IS... 

The neutral lipid fraction from the DEAE-Sephadex A-50 column was combined with the lower phase obtained after Folch partition of the total lipid extract and the combined lipids dried. To the same flask, 10Q ml of 0.6 M NaOH in methanol was added. The mixture was incubated at 37°C for 5 hours. Five volumes of acetone were then added and stored overnight at 4°C. The precipitate was collected by centrifugation at 4°C and dissolved in C M (4 1, v/v). After application to the column (2.0 x 25 cm), the column was washed with chloroform. Neutral glycolipids were then eluted with tetrahydrofuran H2O (10 1). Fractions containing neutral glycosphingolipids were pooled and their glycolipid content examined by thin-layer chromatography. 

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