Cellulytic enzymes

Several combinations of pectinolytic and cellulytic enzymes were tested for their ability to give a cloudy apple juice of Belle de Boskoop apples. Selected results are shown in table I. 

Pew found that the residue, mainly lignin, from unground wood digested by cellulytic enzymes was insoluble in IN sodium hydroxide while the residue from finely ground wood was freely soluble 26). Also, spruce periodate lignin, normally insoluble in methyl cellosolve and in 0. N... 

Cellulose with Cellulytic Enzymes, /. Agric. Food Chem. 1972, 20(6), H73. 

The activity of the cellulytic enzymes was measured as filter paper activity units. A1 x 6 cm strip of Whatman No. 1 filter paper was added to a total volume of 1.5 mL of enzyme solution containing 0.05 M citrate buffer, pH 4.8. All enzyme activity measurements were carried out in triplicate. The samples were incubated for 1 h at 50°C. Reducing sugars were determined after stopping the hydrolysis by adding 3 mL of dinitrosalicylic acid solution followed by boiling for 5 min. After cooling, 20.0 mL of distilled water was added and the absorbance was read at 540 nm (21). 

Pew JC (1957) Properties of powdered wood and isolation of lignin by cellulytic enzymes Tappi 40 553-558... 

So-called cellulytic enzymes are able to reduce the molecular weight once they are present. The point of enzymatic attack is those glycosidic linkages adjacent to unsubstituted anhydroglucose units. The enzymes are able to break these linkages, resulting in viscosity loss (Fig. 5). 

The development of manufacturing processes for stable cellulytic enzymes has resulted in United States patents 3,990,945 (enzymatic hydrolysis of cellulose Huff and Yata 1976), 5,275,944 (Thermostable purified endoglucanase from Acidothermus cellulolyticus Himmel et al. 1994), and 5,712,142 (Method for increasing thermostability in cellulase enzymes Thomas et al. 1997). 

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