Acidothermus cellulolyticus

Acidothermus cellulolyticus cellulase activity, 334,335/,341,342/ cellulase production parameters, 341,343r cellulase synthesis regulation, model development, 341-346 concentration vs. rate of cellulase synthesis, 344,345/... 

Effects of Ammonia Fiber Explosion Treatment on Activity of Endoglucanase from Acidothermus cellulolyticus in Transgenic Plant... 

Index Entries Ammonia fiber explosion endoglucanase Acidothermus cellulolyticus transgenic tobacco Elcd. 

The third example in this chapter is about cellulase, Cel5A from Acidothermus cellulolyticus [69], Its crystal structure complexed with a natural substrate cellote-traose was resolved in 1996. Since then, the protein engineering study around this enzyme has attracted much attention. Especially, one of its mutants, Y245G, was found to increase the catalytic activity about 20 %, when combined with Cel7A. Indeed, one of the bottlenecks for effective biomass conversion is the low catalytic ability of cellulases. Theoretical investigations were carried out to understand the catalytic process using the hybrid QM/MM scheme. 

Sakon L, Adney W.S., Himmel M.E., Thomas S.R. and Karplus P.A. 1996. Crystal structure of thermostable family 5 endocellulase El from Acidothermus cellulolyticus in complex with cellotetraose. Biochemistry 35 10648-10660. 

The development of manufacturing processes for stable cellulytic enzymes has resulted in United States patents 3,990,945 (enzymatic hydrolysis of cellulose Huff and Yata 1976), 5,275,944 (Thermostable purified endoglucanase from Acidothermus cellulolyticus Himmel et al. 1994), and 5,712,142 (Method for increasing thermostability in cellulase enzymes Thomas et al. 1997). 

Class Actinobacteria Subclass Actinobacteridae Order Actinomycetales Suborder Frankineae Family Acidothermaceae Acidothermus cellulolyticus t Acidic hot spring Eppard et al. (1996), Marechal et al. (2000)... 

C. E. Skopec, M. E. Himmel, J. F. Matthews and J. W. Brady Energetics for displacing a single chain from the surface of microcrystalline cellulose into the active site of Acidothermus cellulolyticus Cel5A. Protein Eng. 16, 2003, 1005. 

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