Cellulose affinity

The classical column procedures for the preparation of lysozyme are difficult to use with high viscosity solutions such as serum. A batch method was developed based on affinity chromatography using deami-nated chitin [82]. The method was found to give a one-step purification of nearly theoretical amounts in tissue homogenates tested. These included tissue homogenates from humans, primates, avian egg white and plants. The use of a chitin-coated cellulose affinity column for purification of lysozyme has also been described [83]. 

Kubota N, Nakagawa Y Eguchi Y. Recovery of serum proteins using cellulosic affinity membrane modified by immobilization of Cu + ion. J. Appl. Polym. ScL 1996 62 1153-1160. 

Barroso T, Temtem M, Hussain A, Aguiar-Ricardo A, Roque ACA. Preparation and characterization of a cellulose affinity membrane for human immunoglobulin G (IgG) purification. J. Membr. Sci. 2010 348 224-230. 

Figure 6 Molecular loigth is the major feature controlling cellulose affinity for this series of disperse azo dyes (Ref. 124). Beneficial CoMFA stmc fields ate shown, in orthogonal views, for the most active compound.

SVR applied to mathematical modeling of cellulose affinity of dyestuff... 

The cellulose affinity in dyeing process is another important property of dyestuff. The dyeing of textile fibers led to many theories, which tried to explain the mechanism of dye uptake. One of the most interesting topics is the relationships between the molecular structure and the cellulose affinity of dyestuffs [129]. Table 8.6 lists the data of cellulose affinity of 21 diazo dyes and the following kinds of molecular parameters of these dye molecules the length of the conjugated chain (n), the van der Waals volume (Vw), the dipole moment (p), the HOMO by PM3 method of quantum chemical calculation and six steric parameters ( i, A.2, A.3 and a,... 

Table 8.6 Cellulose affinity and molecular descriptor for diazo dyes.

Using SVR-based feature selection, the most important parameters affecting the cellulose affinity of diazo dyes selected are the length of conjugate chain (n), HOMO and steric parameters. 

Fig, 8.8 Comparison between the values of experimental cellulose affinity and the values predicted by SVR. 

TDTase can be purified by means of various conventional chromatographies (40), oligo(dT)7-cellulose affinity chromatography (6), and an anti-TDTase IgG-Sepharose immunoadsorbent column chromatography (46,55). TDTase is quite stable at pH 4.5, thus providing one of the most useful steps in the purification. 

Biotin-UTP is a biotinylated analog of UTP containing a biotin molecule covalently bound to the C-5 position of the uracil ring via various linkers, e.g., allyla-mine linker arm, Cj i linker (BRL), and C4 or 12-atom linker containing a cleavable disulfide bond (34). Compared with the unmodified UTP, the biotin-C4-UTP is incorporated with an almost equal efficiency, while the biotin-Cu-UTP is incorporated with 50% efficiency. As few as 10 biotin-C4-UMP residues incorporated per 486-nt transcript result in >90% binding efficiency on a streptavidin/biotin-cellulose affinity column (34). 

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