Magnetic cell separation

Separation applications Separation cell Separation, liquid-liquid Separation, low energy Separation, magnetic Separation nozzle Separation of fatty acids Separations... 

Immobilization and recovery of fusion proteins and B-lymphocyte cells using magnetic separation DNA Cell Biol. 12, 191—197... 

Miltenyi, S, Muller, W, Weichel, W, and Radbruch, A (1990) High gradient magnetic cell separation with MACS Cytometry 14, 231-238... 

Kleine, H. D., Schienichen, Lux, E., Pohwoda, H, and Freund, M. (1993) A constant flux system using magnetic cell separator (MACS) for highly efficient T-cell depletion Anal Cell Pathol 5,125—128... 

Pflueger, E., Mueller, E. A, and Anderer, F. A. (1990) Preservation of cytotoxic function during multicycle immunomagnetic cell separations of human NK cells using a new type of magnetic bead J Immunol Methods 129, 165—173... 

Dyer, P. A., Brown, P., and Edward, R. (1998) Immunomethods magnetic, column and panning techniques, in Cell Separation A Practical Approach (Fisher, D., Francis, G. E., and Rickwood, D., eds.), Oxford University Press, Oxford, p. 191. 

Early experimentation on magnetic cell separation was conducted on phagocytic cells (1) and red blood cells (2). Currently, the literature devoted to magnetic cell separation has reached a few thousand publications and shows the diverse benefits that the technology has made to life sciences and clinical research during the past 15 yr... 

Kantor, A. B., Gibbons, I., Miltenyi, S., and Schmitz, J. (1998) Magnetic cell sorting with colloidal superparamagnetic particles, in Cell Separation Methods and Applications (Recktenwald, D., and Radbruch, A., eds.), Marcel Dekker, Inc., New York, pp. 153. 

A magnetic cell separator was constructed on a Si wafer to separate cells that were labeled with paramagnetic beads (FeO nanocrystals 50 nm) from unlabeled ones. The magnetic force was generated from thin magnetized wires (10 pm wide, 0.2 pm thick) formed by depositing a cobalt-chrome-tantalum alloy in pre-etched 0.2-pm-deep trenches. These wires were parallel and were oriented at 45° to the hydrodynamic flow direction of cells [278]. 

Berger, M., Castelino, J., Huang, R., Shah, M., Austin, R.H., Design of a micro-fabricated magnetic cell separator Electrophoresis 2001, 22, 3883-3892. 

Figure 1. A schematic illustration of the magnetic cell separation process.

Magnetic carriers (MC) can be used in biochemistry and biotechnology for cell separation, immobilization of enzymes and other biologically active compounds [1,2]. The use of MC is particularly important for extracorporeal blood purification [3 - 5],... 

Owen CS (1982) Magnetic cell sorting. In Cell Separation Methods and Applications, Vol. 2, TG Pretlow and TP Pretlow, Eds. Academic Press, Orlando, FL. 

Fig. 4. Magnetic cell separation. Left) Traditional method using large magnetic particles to bind to the cells. Right)The use of magnetic nanopartides that interact with the cell surface and thereby form large segregates that csm be collected with a magnetic trap.

MACS magnetic cell separation NCX NaKCa " exchanger... 

Kuhara M, Takeyama H, Tanaka T, Matsunaga T (2004) Magnetic cell separation using antibody binding with protein a expressed on bacterial magnetic particles. Anal Chem 76 6207-6213... 

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