Cell membranes microdomains

Fyn is a nonreceptor tyrosine kinase related to Src that is frequently found in cell junctions. Die protein is N-myristoylated and palmitoylated and thereby becomes associated with caveolae-like membrane microdomains. Fyn can interact with a variety of other signaling molecules and control a diversity of biological processes such as T cell receptor signaling, regulation of brain function, and adhesion mediated signaling. 

Figure 23 Representation of a cell membrane according to the fluid mosaic model (Singer, 1974). In this model, the aqueous phospholipid interfacial microdomain separates the water compartment from the apolar membrane interior. [Redrawn from Burton et al. (1992) with permission from the publisher.]...

Zacharias, D. A., Violin, J. D., Newton, A. C. and Tsien, R. Y. (2002). Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. Science 296, 913-6. 

K. E. Pace, C. Lee, P. L. Stewart, and L. G. Baum, Restricted receptor segregation into membrane microdomains occurs on human T cells during apoptosis induced by galectin-1, J. Immunol., 163 (1999) 3801-3811. 

The lipid compositions of plasma membranes, endoplasmic reticulum and Golgi membranes are distinct 26 Cholesterol transport and regulation in the central nervous system is distinct from that of peripheral tissues 26 In adult brain most cholesterol synthesis occurs in astrocytes 26 The astrocytic cholesterol supply to neurons is important for neuronal development and remodeling 27 The structure and roles of membrane microdomains (rafts) in cell membranes are under intensive study but many aspects are still unresolved 28... 

Babiychuk EB, Draeger A 2000 Annexins in cell membrane dynamics. Ca2+-regulated association of lipid microdomains. J Cell Biol 150 1113-1124 Ber DM 2001 Excitation-contraction coupling and cardiac contractile force, 2nd edn. Kluwer Academic Publishers, Dordrecht/Boston/London Blaustein MP, Golovina VA 2001 Structural complexity and functional diversity of endoplasmic reticulum Ca2+ stores. Trends Neurosci 24 602—608 Flynn ER, Bradley KN, Muir TC, McCarron JG 2001 Functionally separate intracellular Ca2+ stores in smooth muscle. J Biol Chem 276 36411-36418 Fry CH, WuCl 997 Initiation of contraction in detrusor smooth muscle. Scand J Urol Nephrol Suppl 184 7-14... 

Y. Lavie, G. Fiucci, M. Czarny, and M. Liscovitch. Changes in membrane microdomains and caveolae constituents in multidrug-resistance cancer cells. 

Thomsen P, Roepstorff K, Stahlhut M, van Deurs B. Caveolae are highly immobile plasma membrane microdomains, which are not involved in constitutive endocytic trafficking. Mol Biol Cell 2002 13(l) 238-250. 

Cordeaux Y, Briddon SJ, Alexander SP, Kellam B, Hill SJ (2008) Agonist-occupied A3 adenosine receptors exist within heterogeneous complexes in membrane microdomains of individual living cells. FASEB J 22(3) 850-860... 

Maxfield FR. Plasma membrane microdomains. Curr Opin Cell Biol. 2002 14 483-487. 

Lipids have several important functions in animal cells, which include serving as structural components of membranes and as a stored source of metabolic fuel (Griner et al., 1993). Eukaryotic cell membranes are composed of a complex array of proteins, phospholipids, sphingolipids, and cholesterol. The relative proportions and fatty acid composition of these components dictate the physical properties of membranes, such as fluidity, surface potential, microdomain structure, and permeability. This in turn regulates the localization and activity of membrane-associated proteins. Assembly of membranes necessitates the coordinate synthesis and catabolism of phospholipids, sterols, and sphingolipids to create the unique properties of a given cellular membrane. This must be an extremely complex process that requires coordination of multiple biosynthetic and degradative enzymes and lipid transport activities. 

Zhang, W., Trible, R. P., and Samelson, L. E. 1998. LAT palmitoylation Its essential role in membrane microdomain targeting and tyrosine phosphorylation during T cell activation. Immunity 9 239-246. 

Lagerhokn BC, Weinreb GE, Jacobson K, Thompson NL. Detecting microdomains in intact cell membranes. Annu. Rev. Phys. 

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