Cell-killing effect

LCso - concentration providing 50% cell killing effect CV - crystal violet coloration. 

Err 1080 - hnman fibrosarcoma MG-22A - monse hepatoma LC50 - concentration providing 50% cell killing effect CV - crystal violet coloration MTT - 3 (4,5 dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolinm bromide coloration. 

ICso [pg/mL] providing 50% cell killing effect (CV coloration MTT coloration) crystal violet (CV),... 

The spatial separation of the chloride ions in the czVplatinum complexes, 0.33 nm, and those of the chlorides at the ends of the chloroethyl arms of the nitrogen mustard, 0.80 nm, suit the formation of intra- and interstrand bridges, respectively. The cell-killing effect results from an inability, or very much decreased ability, of tumour cells to repair the intrastrand breaks. 

The cell-killing effects of cisplatin (and carboplatin) appear to be due to the formation of various stable bifunctional adducts on DNA, which then block replication or inhibit transcription. Supportive evidence for DNA as the critical target for the antitumour activity of cisplatin is provided by observations that cells from patients with diseases where DNA repair processes are deficient e.g. Xeroderma pigmentosum) are hypersensitive to cisplatin. In addition, correlations have been shown between levels of platinum-DNA adducts in peripheral blood cells (leukocytes) and disease response in patients receiving cisplatin or carboplatin. ... 

The combination of radiotherapy and chemotherapy is a promising approach because of its independent cell kill effect and the property of some cytotoxic agents to enhance the effect of radiotherapy. At the end of the 1970s, platinum complexes were described as being able to act as potent radiosensitizers of hypoxic tumor cells (Dou-PLE and Richmond 1978,1979). As an obstacle in this type of chemoradiation, Koukourakis etaL (2002) have suggested that (hypoxia-induced ) overexpression of HIF-1 a in patients with head and neck cancer may be related to substantial resistance to carboplatin chemo-radiotherapy. More in-depth research is needed to accurately characterize adverse effects of hypoxia in this type of combination therapy. 

In Vitro GdNCT 11.2.2.1 Tumor Cell Killing Effect... 

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