Hematopoietic stem cells , cell-based therapies

Su L, Lee R, Bonyhadi M, etal. Hematopoietic stem cell-based gene therapy for acquired immunodeficiency syndrome efficient transduction and expression of RevM 10 in myeloid cells in vivo and in vitro. Blood 1997 89(7) 2283-2290. 

Bosi A, Bartolozzi B, Vannucchi AM, Orsi A, Guidi S, Rossi Ferrini P. Polymerase chain reaction-based pre-emptive therapy with cidofovir for cytomegalovims reactivation in allogeneic hematopoietic stem cells transplantation recipients a prospective study. Haematologica 2002 87(4) 446-7. 

Haln T, Wolff SN, Czuczman M, et al. The role of cytotoxic therapy with hematopoietic stem cell transplantation in the therapy of diffuse large cell B-cell non-Hodgkin s lymphoma an evidence-based review. Biol Blood Marrow Transplant 2001 7 308-331. 

To circumvent this problem, vectors that are based on lentiviruses have been developed. In contrast to prototypic retroviruses, lentiviruses do not require cell division for integration. Gene-therapy vectors have been developed from a broad spectrum of lentiviruses including human immunodeficiency vims (HIV), simian and feline immunodeficiency vims as well as visna/maedi vims. The most widely used lentiviral vector system is based on HIV-1. These vectors can efficiently transduce a broad spectrum of dividing and nondividing cells including neurons, hepatocytes, muscle cells, and hematopoietic stem cells [1,2]. 

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