Cell Adhesion and Detachment

Tsuda Y, Kikuchi A, Yamato M et al (2004) Control of cell adhesion and detachment using temperature and thermoresponsive copolymer grafted culture surfaces. J Biomed Mater Res A 69 70-78... 

Dictyostelium has been shown thus far to only have relatively weak contacts with the substratum. Observations by interference reflection microscopy show that highly chemotactic Dictyostelium cells have relatively small areas of contact with the substratum [193, 231]. They do not demonstrate the focal contacts that are seen with mesenchymal cells. The contact areas with the substratum do not show focal accumulations of cytoskeletal proteins, consistent with the lack of focal contacts. Flow force measurements of cell adhesion and detachment show that mutations in cytoskeletal proteins can reduce the adhesion strength [55, 161, 208, 221]. Dictyostelium cells can form eupodia on their dorsal surfaces when in contact with agarose on that surface [74, 75]. These structures may represent more localized adhesion sites, which are present when the appropriate substratum is available. In addition, during aggregation, chemotactic responses may be modified by cell-cell adhesion. 

Cell Adhesion and Detachment, Fig. 1 Ch ical reaction scheme for the attachment of any molecule with a primary amine group to a silicon, glass, or PDMS surface... 

Cell Adhesion and Detachment, Fig. 3 Selective adhesion of T lymphocytes in a microfluidic device as a function of shear stress. The device geometry, shown in blue, is such that the fluid shear stress decreases along the length of the device. This device was coated with the... 

Cell Adhesion and Detachment, Fig. 4 A self- converted into a lactone group upon the application of an... 

Cell Adhesion and Detachment, Fig. 5 Release of cells from a surface coated with self-assembled mono-layers coupled to the RGD peptide. The left side of the surface has a monolayer that is not electroactive, and the right side of the surface has a monolayer that contains the electroactive quinone group. Fibroblast cells initially adhere on both sides of the surface (a). Upon the application of an electrical potential, the quinone groups are converted to lactone groups and release the RGD groups along with the cells attached to these groups (b) (Taken from Yeo et al. [3])... 

Cell Adhesion and Detachment, Fig. 6 Cell detachment using the thermally responsive polymer PNIPAM. A surface coated with the polymer is seeded with human keratinocytes cells which form a sheetlike layer... 

Cell Adhesion and Detachment, Fig. 7 Biomolecule release within a microfluidic device using PNIPAM films and microfahricated heaters (a). The release of... 

Mechanical responses of cells to physical or chemical stimuli include cell growth patterns, morphology, adhesion, and motility. Many mammalian cells grown in culture are adherent. For such cells, the adhesive interactions with the physical environment (extracellular matrix or other cells) are a critical determinant of their biochemistry and behavior. Microfluidics has become a useful tool for studying these interactions. Many of these studies are reviewed elsewhere ( Cell Adhesion and Detachment), so the focus here is on assays that evaluate the effects of physical or chemical cues on cell morphology or motility. 

Cell Adhesion and Detachment Cell Assays in Microfluidics Cell Patterning on Chip Droplet Microreactor... 

Yoon, S. H. Mofrad, M. R. K. Cell adhesion and detachment on gold surfaces modified with a thiol-functionalized RGD peptide. Biomaterials 2011, 32, 7286-7296. 

Cell Adhesion and Detachment, Figure 2 Chemical reaction scheme for the attachment of a biomolecule to the surface of polymeric microfluidic devices made of PMMA or PC. The carbodiimide coupling process starts with a reaction with carboxyiic acid groups on the surface... 

Cell Adhesion and Detachment, Figure 4 A self-assembled monolayer with an electroactive functional group bound to the RGD peptide. This quinine group is converted into a lactone group upon the application of an electrical potential and this change releases the RGD group from the surface. Taken from [3]... 

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