CDC2 kinase

Figure 2. Regulators of the Cdc2 protein kinase. Schematic illustration of the regulators of Cdc2 kinase activity. Proteins that are circled indicate proteins that have been identified in higher eukaryotes only the squared proteins have been identified exclusively in the yeast systems. The remaining proteins have been identified in both higher and lower eukaryotes. Protein phosphatases are indicated by p tase.

Kipreos, E. T., and Wang, J. Y. J. (1990). Differential phosphorylation of c-abl in cell cycle determined by cdc2 kinase and phophatase activity. Science 248 217-220. 

Lorca, T., Labbe, J-C., Devault, A., Fesquet, Capony, J-P., Cavadore, J-C., Le Bouffant, F., and Doree, M. (1991). Dephosphorylation of cdc2 on threonine is required for cdc2 kinase inactivation and normal anaphase. EMBO J. 11 2381-2390. 

Nigg, E. A. (1991). The substrates of the cdc2 kinase. Semin. Cell Biol. 2 261-270. 

Yasuda, H., Nakata, T., Masayuki, M., Honda, R., Nakamura, M Ninomija-Tsuji, J., Yamashita, M., Nagahama, Y., and Ohba, Y. (1992). Cyclin-dependent kinase 2 (cdk2) in the murine cdc2 kinase TS mutant Som. Cell Mol. Gen. 18 403-408. 

Mailer We think S phase suppression after anaphase I is just a secondary effect of having significant Cdc2 kinase activity still around. The MAP kinase pathway is unable to suppress S phase in the absence of cyclin B. 

Nasmyth It probably has nothing to do with Cdc2 kinase. 

Cdc2 kinase activity is required during mitosis but not during interphase for apical Insc localisation... 

Sigrist S, Ried G, Lehner CF 1995 Dm cdc2 kinase is required for both meiotic divisions during Drosophila spermatogenesis and is activated by the Twine/Cdc25 phosphatase. Mech Dev 53 247-260... 

A. Goldbeter, A minimal cascade model for the mitotic oscillator involving cyclin and cdc2 kinase. Proc. Natl. Acad. Sci. USA 88, 9107-9111 (1991). 

Phosphorylation of HI during mitosis is catalyzed by the cyclin B-Cdc2 kinase, a tightly regulated enzyme [25,26]. In Tetrahymena mitotically dividing nuclei, cyclic-AMP dependent kinase (PKA) or PKA-like kinase phosphorylates HI [27,28]. Protein phosphatase 1 dephosphorylates the phosphorylated HI [29]. 

CDC2 kinases (representatives of this family are central elements of regulation of the cell cycle, see Chapter 13... 

Entry into and the course of mitosis are primarily determined by the activity of the CDC2 kinase. The CDC2 kinase in the active form exists as a complex with cychn B and, together with the cyclin, forms the mitosis promoting factor, MPF. The activity of MPF oscillates in the cell cycle and is the triggering factor for entry of the cell into M phase. 

Morishita, R., Gibbons, G.H., Ellison, K.E., et al. (1993). Single intraluminal delivery of antisense cdc2 kinase and proliferating-cell nuclear antigen oligonucleotides results in chronic inhibition of neointimal hyperplasia. Proc. Natl. Acad. Sci. U.S.A., 90, 8474-8478. 

Microxine (489), a purine derivative was isolated from the Australian marine sponge Microxina sp. Microxine was found to weakly inhibit Cdc2 kinase activity [417]. 

Peter M, Heitlinger E, Haner M, Aebi U, Nigg EA. 1991. Disassembly of in vitro formed lamin head-to-tail polymers by CDC2 kinase. EMBO J 10 1535-1544. 

Lowe M, Rabouille C, Nakamura N, Watson R, Jackman M, Jamsa E, Rahman D, Pappin DJ, Warren G. Cdc2 kinase directly phosphorylates the cis-Golgi matrix protein GM130 and is required for Golgi fragmentation in mitosis. Cell 1998 94 783-793. 

Peter M, Nakagawa J, Doree M, Labbe JC, Nigg EA. In vitro disassembly of the nuclear lamina and M phase-specific phosphorylation of lamins by cdc2 kinase. Cell 1990 61 591-602. 

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