Cassette accelerated rapid rat screening

There are a number of solutions that have been proposed to address the limitations on throughput in PK assays, including cassette dosing [37], where typically five compounds are dosed in a mixture, pooling of plasma samples from multiple animals receiving a specific dose, or the cassette-accelerated rapid rat screen where the processing of samples is streamlined [38]. 

K. J. Veals, J. Hsieh, Y. etal. Cassette-accelerated rapid rat screen a systematic procedure for the dosing and liquid chromatography/ atmospheric pressure ionization tandem mass spectrometric analysis of new chemical entities as part of new drug discovery. Rapid Commun Mass Spectrom 2001, 15, 335-340. 

Figure 1.4. NCE/NME progression scheme showing the various discovery stage liquid chromatography-mass spectrometry (LC-MS) and LC-tandem MS (LC-MS/MS) assays used for selecting NME/NCE to advance into development. (Reprinted with permission from Korfmacher, 2005.) (CARRS, Cassette accelerated rapid rat screening IV, Intravenous administration PO, Oral administration NCE, New chemical entity)...

Korfmacher and co-workers have increased efficiency through streamlined and standardized rehnement of the bioanalytical process [119]. Their approach, known as cassette-accelerated rapid rat screen (CARRS), is based around a 96-well template that accommodates six compounds dosed via single-compound administration. Higher efficiency is achieved through the use of abbreviated standard curve.s (25, 250, and 2500 ng/mL), time points that are pooled from multiple animals, and the use of a standardized format. 

Korfmacher, W.A. Cox, K.A. Ng, K.J. Veals, J. Hsieh, Y. Wainhaus, S. Broske, L. Prelusky, D. Nomeir, A. White, R.E. Cassette-Accelerated Rapid Rat Screen A Systematic Procedure for the Dosing and Liquid Chromatography/Atmospheric Pressure Ionization TandemMass Spectrometric Analysis of New Chemical Entities as Part of New Drug Discovery, Rapid Commun. Mass Spectrom. 15, 335-340 (2001). 

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