Caseins beads

FIAs can be based on steady-state intensity measurements without probe amplification, owing to the sensitivity of detection that is possible with fluorescence instrumentation, which exceeds that of spectrophotometers by two or three orders of magnitude. A sensitive fluorometer has been described for an estradiol assay(36) in which the limit of estradiol detection is 3 x KT11 M. Estradiol antibody labeled with rhodamine B is reacted with estradiol samples. Unreacted labeled antibody is removed with Sepharose-estradiol-casein beads, and the remaining fluorescence is directly proportional to the analyte concentration. The detection limit of rhodamine B on the same fluorometer is 5 x 1(T12 M. This instrument uses a 0.75 mW green helium-neon (HeNe) laser to irradiate the sample from above, at the air-liquid interface, to increase the light path and to decrease surface reflections. The sample compartment has a top-mounted photon trap, and a mirror mounted on the side of the sample compartment opposite the PMT to enhance detection. 

Sodium caseinate or calcium caseinate can be used for the preparation of casein beads by acidic precipitation. Green tea polyphenols have been encapsulated in such beads with a positive effect on the preservation of the antioxidant properties. ... 

Dehkharghanian M, Lacroix M, Vijayalakshmi MA. Antioxidant properties of green tea polyphenols encapsulated in caseinate beads. Dairy Sci Technol. 2009 89(5) 485-499. 

It was found that cytochrome c, (J-casein, and BS A can be digested by trypsin-immobilized beads in a microchamber on a glass chip. The protein (1 mg/mL) was mobilized by EOF. Although without external heating, Joule heating may assist in the enzymatic digestion reaction of the proteins [116]. 

Heth and Swaisgood (1982) examined the preferential binding of caseins to thioester-derivatized glass beads. The micelles were first separated into micellar and serum casein fractions by chromatog-... 

Neeser, J.R., Golliard, M., Woltz, A., Rouvet, M., Dillmann, M.L., and Guggenheim, B. 1994. In vitro modulation of oral bacterial adhesion to saliva-coated hydroxyapatite beads by milk casein derivatives. Oral Microbiol. Immunol. 9, 193-201. 

Bulgarelli, E., Fomi, F. and Bemabei, M.T. 1999. Casein gelatin beads I. Cross-linker solution composition effect on cross linking degree, 190 175-182. 

Farmingdale, USA) combined with glass beads. Supernatants from lysed cells are examined for enzyme activities believed to be of importance for flavor development in cheese. These include a variety of aminopeptidase and aminotransferase activities that are involved in the conversion of the peptides and amino acids derived from the breakdown of casein to a variety of flavor compounds. 

Microsphere-filled capillary microreactor TPCK-treated trypsin Covalent linking Magnetic beads with carboxylic acid functionalized surface immobilization of trypsin onto beads via EDC activation microreactor coupled to CE-ESI-MS for digestion of insulin chain b and P-casein, that is, a 2-dimensional CE system. Platform delivers tryptic digests to the MS within a short window [132]... 

---