Case Study 3 Method Development for a Diprotic Basic Compound

6 Case Study 3 Method Development for a Diprotic Basic Compound 

Step 2 Determine the isocratic conditions for pH scouting studies. 

A) Determine the optimal pH of the aqueous portion of the mobile phase for gradient scouting studies in Step 5. (paper based evaluation) 

Step 5 Gradient scouting studies (shallow/steep x 2 temperatures) with the optimized pH of the aqueous phase use crude and forced degraded samples 

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