Carrot pectin isolation

During the course of bile acid binding assays of carrot AAIR it was noted that a peak emerged at the void volume during HPLC of the solution of bile acid that had been in contact with the fiber. We therefore suspected that the bile acid solution was solubilizing some small fraction of the AAIR. Accordingly, carrot AAIR was then extracted with sodium deoxycholate and a pectin fraction was isolated by alcohol precipitation of the... 

Problems In the extraction, isolation, fractionation, and subsequent analysis of non-starchy polysaccharides, which may function as dietary fibre components, are discussed for such substances from carrots and apples. Structural studies on polysaccharides from carrots have resulted In the characterization of a branched arablnan, an esterlfled galacturonan (pectin), and an acidic iqrlan as individual components, and further evidence points to the presence of a 4 -linked galactan and a 4-linked glucomannan. 

Preparation of Apple CWM and AIR and Extraction of Pectin Fractions. Apples ( Granny Smith variety) were cored and peeled and the resulting pulp (2 kg batches) was stored in ethanol suspension to minimize enzymic oxidation. Separate batches of pulp were processed, as described for carrot preparations, to furnish apple CWM (32.5 g) and apple AIR (39.8 g) for which analytical data are recorded in Table VI. Polysaccharide fractions B (4 g) and C (2.5 g) were extracted from CWM with water at 80° and aqueous 1% ammonium oxalate at 80°. Likewise polysaccharide fractions A (2.1 g), B (5.2 g), and C (4.5 g) were Isolated from AIR by extraction with water at room temperature, 80°, and with aqueous 1% ammonium oxalate... 

---