Carnoy’s solution

Carnoy s solution (v/v) 6 absolute ethanol/3 chloroform/1 glacial acetic acid... 

One day (Pll), 7 days (P17), and 20 days (P30) after drug injection, treated and untreated control rats of the same age are deeply anesthetized and euthanized. The brains are quickly removed, fixed in Carnoy s solution for 48 h, then placed in absolute ethanol for 50 min and in acetone for 30 min, and embedded in Paraplast X-tra. Paraplast-embedded sections of the cerebellar vermis are obtained parallel to the sagittal plane. 

A high speed hybridization procedure (total, including fixation and detection, 1 h) has been described by Bourinbaiar et al. (1991). They suspended washed lymphocytes, for the detection of HIV, in a mildly hypotonic medium (1 part medium and 2 parts distilled water) and spread 25 xl ( 10 cells) in 8 mm wells of Teflon-coated multiwell glass slides and fixed the cells in Carnoy s II solution (60% methanol, 30% chloroform, 10% glacial acetic acid) for 10 min at room temperature and then for 10 min in absolute acetone. Five microliters of biotinylated probes (100 ng/ml) is added to the dried cells and the slides are covered with autoclavable plastic (cut from biohazard bag) and placed in a microwave oven. The position of the slide in the oven and the incubation time are crucial for this hybridization step. In microwave ovens with a carousel, the slide is placed about 6 cm from the center and a 150 ml beaker filled with tap water is placed in the center. The slide is then irradiated for 30 s on Defrost . The probes can be detected after a rinse with 2 x SSC. 

Methacarn Methanol Carnoy s fixative Freshly prepare, by volume, a solution of 60% methanol, 30% chloroform and 10% glacial acetic acid. 

Methacarn is a fixative used on tissues to be paraffin-embedded and immunochemically stained in which methanol has been substituted for the ethanol in Carnoy s fluid. This methanol-Carnoy mixture is referred to as methacarn solution. It preserves certain tissue and cell components such as collagen and cytoskeletal elements exceptionally well for immunoperoxidase and other immunochemical staining (8). 

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