Carcinogens DNA adducts

If a-hydroxylation of NPYR is its mechanism of activation, one would expect the formation of carcinogen-DNA adducts containing a 4-oxobutyl- or related residue. Adducts have been isolated from the liver RNA of NPYR treated rats, but their structures have not been determined (25). Carcinogen DNA adducts have also been isolated from cultured human esophagus, colon, and bronchus (26, 27, 28). 

The role of N-acetoxy arylamides as metabolically formed ultimate carcinogens jji vivo also appears to be limited. Their enzymatic formation via peroxidation of N-hydroxy arylamides can be excluded since tissues containing high levels of peroxidases such as the rat mammary gland (83) and the dog urinary bladder (84) do not form acetylated carcinogen-DNA adducts in vivo (63). Their non-enzymatic formation by reaction of acetyl coenzyme A with N-hydroxy arylamides (6 ) cannot be excluded however, even if formed, their direct reaction with cellular DNA appears unlikely as treatment of cultured cells with synthetic N-acetoxy AAF (85,86) results primarily in deacetylated arylamine-DNA adducts, apparently due to rapid N-deacetylation to form the reactive N-acetoxy arylamine (V). 

In a recent procedure to detect carcinogen-DNA adducts by radioimmunoassay, A-(guanosin-8-yl)acetylaminofluorine was linked to BSA by the periodate method, and the conjugate was used to elicit specific antibodies to this product, which is formed when N-acetoxy-2-acetylamino-fluorene reacts with DNA. 

See a/so Analytical Toxicology Biomarkers, Environmental Carcinogen-DNA Adduct Formation and DNA Repair ... 

Carcinogen-DNA adducts are addition products formed by covalent binding of all or part of a carcinogen molecule to chemical moieties in DNA adducts are formed when an activated chemical species (electrophilic, positively charged metabolite) binds covalently to negatively charged moieties in DNA. 

Measurement of Carcinogen-DNA Adducts as Human Exposure Dosimeters... 

See also Carcinogen Classification Schemes Carcinogen-DNA Adduct Formation and DNA Repair Cell Proliferation Chromosome Aberrations Epidemiology Immune System International Agency for Research on Cancer Mechanisms of Toxicity Molecular Toxicology-Recombinant DNA Technology Mouse Lymphoma... 

See also Ames Test Analytical Toxicology Carcinogen Classification Schemes Carcinogen-DNA Adduct... 

See also Androgens Carcinogen-DNA Adduct Formation and DNA Repair Chromosome Aberrations Deveiop-mentai Toxicoiogy Dose-Response Reiationship Endocrine System Epidemioiogy Reproductive System, Maie Risk Assessment, Human Heaith Sister Chromatid Exchanges Toxicity Testing, Deveiopmentai Toxicity Testing, Reproductive. 

See also Ames Test Analytical Toxicology Aneuploidy Carcinogen-DNA Adduct Formation and DNA Repair Chromosome Aberrations Developmental Toxicology Dominant Lethal Tests Host-Mediated Assay Molecular Toxicology - Recombinant DNA Technology Mouse Lymphoma Assay Toxicity Testing, Mutagenicity. 

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