Molecular Size of Carbohydrates by Zone Electrophoresis

Determination of Molecular Size of Carbohydrates BY Zone Electrophoresis 

The fact that carbohydrates of different molecular sizes [for example, pentoses and hexoses (see Table I)] may have identical Mg values on subjection to zone electrophoresis (in borate buffers) indicates that this system cannot be used for determining the molecular size of a carbohydrate. It has been emphasized at several points in this Chapter that the most potent use for zone electrophoresis of carbohydrates in borate buffers is after mixtures of carbohydrates have been resolved into groups of similar molecular size by chromatographic procedures. 

Relative Mobilities of Aldoses, as N-Bemylglycosylammonium lons and as Bisulfite Complexes - 

Aldose or derivative Mobility of N-benzylglycosyt-ammonium ion, relative to that of N-henzyl-j glucosylamine Mol. wt. Aldose or derivative Mobility of bisulfite complex, relative to that of n-glucose 

The relative mobilities as determined by each method are listed in Table XI, from which it may be seen that they are related to the molecular weight this permits the determination of the molecular size of an aldose, at least up to a hexasaccharide. The decrease of mobility with increase in molecular weight is not linear. 

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V. Determination of Molecular Size of Carbohydrates by Zone Electrophoresis. 107... 

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