Carbohydrate-protein linkage region structural studies

VIH. Structural Studies on the Carbohydrate-Protein Linkage Region. 215... 

Sugahara K, Yamashina 1, Dewaard P, Vanhalbeek H, Vhegen-thart JFG. Structural studies on sulfated glycopeptides from the carbohydrate-protein linkage region of chondroitin 4-sulfate proteoglycans of swarm rat chondrosarcoma - demonstration of 86. the structure Gal(4-0-Sulfate)-P-l-3-Gal-P-l-4Xyl-P-l-0-Ser. J. 

Carbohydrates are much more difficult to study using ORD and CD than proteins and nucleic acids and much less literature is available. First, carbohydrates mainly absorb below 190 nm, a region difficult to examine using commercial CD spectrometers. Second, unlike proteins and nucleic acids, there are no common sets of spectral characteristics which easily define the conformation of carbohydrates. However, ORD and CD have been used to obtain useful information about the structure of carbohydrates. In favourable circumstances they can give information about the structure and linkages in carbohydrates. In addition, they can distinguish helical from disordered conformations and be used to monitor conformational transitions induced by salts, solvents and temperature. 

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