Capillary isoelectric focusing isotachophoresis

CE provides a complementary approach to HPLC separation. It is performed in several different formats, including capillary zone electrophoresis, miceller electrokinetic chromatography, capillary gel electrophoresis, capillary isoelectric focusing, isotachophoresis, and capillary electrochromatography. Of these formats, capillary zone electrophoresis is the most popular separation technique. The most successful coupling of CE with mass spectrometry is achieved via an ESI interface. The three most practical designs are sheathless interface, sheath-flow interface, and liquid-junction interface. [Pg.186]

CE was recently used for anthocyanin analysis because of its excellent resolution. This technique has different modes capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), micellar electrokinetic chromatography (MEKC), capillary electrochromatography (CEC), capillary isoelectric focusing (CIEE), and capillary isotachophoresis (CITP)."° CZE is the most popular method for anthocyanin... [Pg.489]

Capillary electrophoresis (CE) coupled to MS has the advantage of high resolution and soft ionization for biomolecules, which may be used to differentiate post-translational modifications and variants of intact proteins and oligonucleotides. Different modes of CE (capillary zone electrophoresis, capillary isoelectric focusing, capillary electrochromatography, micellar electrokinetic chromatography, nonaqueous capillary electrophoresis) to MS as well as online preconcentration techniques (transient capillary isotachophoresis, solid-phase extraction, membrane preconcentration) are used to compensate for the restricted detection sensitivity of the CE methodology [77, 78]. [Pg.174]

The second separation mechanism is found in capillary isoelectric focusing (cIEF), where analytes are separated on the basis of isoelectric points. The third mechanism is found in capillary isotachophoresis (cITP), where all of the solutes travel at the same velocity through the capillary but are... [Pg.154]

Other approaches to enhance the sensitivity can be sub-divided to (i) concentration outside the capillary (solid-phase extraction, etc.) and (ii) concentration within the capillary using hyphenated techniques such as isoelectric focusing, isotachophoresis, and on-column stacking. [Pg.94]

Capillary electrophoresis is a general term that is used to describe a number of different separation techniques. Capillary zone electrophoresis (CZE) is the classic technique and is therefore usually referred to as just CE. Other techniques include micellar electrokinetic chromatography (MEKC), capillary isoelectric focusing, and capillary isotachophoresis. CZE and MEKC are the predominant techniques and are those used herein, so only they will be discussed in detail here. [Pg.390]

The main separation modes used in CE are capillary zone electrophoresis (CZE), micellar electrokinetic capillary chromatography (MEKC), capillary isotachophoresis, capillary gel electrophoresis, and capillary isoelectric focusing. CZE and MEKC are used most often. CE buffers are generally aqueous-based, though nonaqueous systems are exploited as well, particularly for analytes that are insoluble or sparingly soluble. [Pg.608]

Capillary electrophoresis is the generic name for a family of related techniques which have their origin in capillary zone electrophoresis (CZE) and are capillary gel electrophoresis (CGE), capillary isoelectric focusing (CIEF), miscellar electrokinetic capillary chromatography (MECC) and capillary isotachophoresis (CITE). Though the techniques differ significantly in principle of operation they can be carried out largely on the same basic instrumentation. [Pg.108]

Mohan D, Lee C S (2002). On-line coupling of capillary isoelectric focusing with transient isotachophoresis-zone electrophoresis A two-dimensional separation system for proteomics. Electrophoresis. 23 3160-3167. [Pg.510]

Try alternative separation mode micellar electrokinetic chromatography (MEKC), capillary isotachophoresis (CITP), capillary isoelectric focusing... [Pg.57]

Note CEC capillary electrochnunatography, CZE capillary zone electrophoresis, CGE capillary gel electrophoresis, CIEF capillary isoelectric focusing, CFTP capillary isotachophoresis, MEKC micellar electrokinetic chromatography... [Pg.273]