Capillary electrophoresis resolving power

Capillary electrophoresis, a powerful high-efficiency high-resolution analytical technique, was used for the separation and characterization of cyclic-AMP, cyclic-GMP, and cyclic-IMP. Reproducibility, linear-ity, and spectral analysis were tested. The results shows that capillary electrophoresis is a reliable technique used to resolve and quantitate sub-picomole amounts of a mixture of cyclic nucleotides. 

Capillary electrophoresis has also been combined with other analytical methods like mass spectrometry, NMR, Raman, and infrared spectroscopy in order to combine the separation speed, high resolving power and minimum sample consumption of capillary electrophoresis with the selectivity and structural information provided by the other techniques [6]. 

The choice of HPLC as a separation technique in biopharmaceutical development may depend upon its scalability, even if its resolving power is less than other techniques. For example, HPLC may be used in preference to gel electrophoresis or capillary electrophoresis, where scaleup is difficult or impossible. 

Capillary electrophoresis is a new technique that combines the high resolving power of electrophoresis with the speed, versatility, and automation of... 

Capillary electrophoresis has found use in the biotechnology industry for structural analysis of recombinant proteins. The high resolving power of CE for charged analytes makes it a powerful tool for the analysis of tryptic digests. Therefore, many of the techniques given here, such as the determination of thiols, carbohydrates, and amino acids, will be employed for this purpose. 

Some residues require derivatization to enhance the extractability, clean-up, or subsequent chromatographic resolution and determination steps. Instead of chromatography, capillary electrophoresis with a high resolving power may be considered as well. 

The advancement of modern biochemistry and developments in micro- and macromolecular separations have been intimately linked. Capillary electrophoresis offers major advantages over other separation techniques, including speed and resolving power. The potential of capillary electrophoresis seems so vast that it will significantly complement the technology of high-performance liquid chromatography. However, because of unique characteristics of capillary electrophoresis, it will also... 

The excellent resolving power of gel or polymer solution-filled narrow-bore channels readily provides sequencing-grade separation of very similar size DNA molecules, usually detected by laser-induced fluorescence [9]. Collection of any individual or all separated fragments by capillary electrophoresis (CE) proved to be feasible and provided enough material for such downstream processes as polymerase chain reaction (PCR), sequencing, or cloning [10-12],... 

Capillary electrophoresis is a technique that can be used to analyze and separate proteins. It has a high resolving power that exceeds other electrophoretic techniques and is capable of distinguishing between proteins that differ only slightly in amino acid composition or glycosylation. 

Capillary electrophoresis (CE) is a powerful separation technique. It is especially useful for separation of ionic compounds and chiral mixtures. Mass spectrometry has been coupled with CE to provide a powerful platform for separation and detection of complex mixtures such as combinatorial libraries. However, the full potential of CE in the application of routine analysis of samples has yet to be realized. This is in part due to perceived difficulty in the use of the CE technique compared to the more mature techniques of HPLC and even SFC. Dunayevskiy et al. [136] analyzed a library of 171 theoretically disubstituted xanthene derivatives with a CE/ESI-MS system. The method allowed the purity and makeup of the library to be determined 160 of the expected compounds were found to be present, and 12 side products were also detected in the mixture. Due to the ability of CE to separate analytes on the basis of charge, most of the xanthene derivatives could be resolved by simple CE-MS procedures even though 124 of the 171 theoretical compounds were isobaric with at least one other molecule in the mixture. Any remaining unresolved peaks were resolved by MS/MS experiments. The method shows promise for the analysis of small combinatorial libraries with fewer than 1000 components. Boutin et al. [137] used CE-MS along with NMR and MS/MS to characterize combinatorial peptide libraries that contain 3 variable positions. The CE-MS method was used to provide a rapid and routine method for initial assessment of the construction of the library. Simms et al. [138] developed a micellar electrokinetic chromatography method for the analysis of combinatorial libraries with an open-tube capillary and UV detection. The quick analysis time of the method made it suitable for the analysis of combinatorial library samples. CE-MS was also used in the analysis... 

To more rapidly process these millions of pieces, the whole operation is done automatically, performing separations by capillary electrophoresis, which has higher resolving power and is faster than gel electrophoresis. Detection is done by passing a laser beam through the eluting drops at the ends of multiple capillaries. The public consortium has largely switched to this instrument as well (the ABI Model 3700). 

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