Candida utilis, conversion

The enzyme Candida utilis 6-phosphogluconate dehydrogenase with carbon-13 enriched to 90 atom% has been obtained by growing the yeast with Relabelled acetic acid as the sole carbon source [186]. Carbon-13 composition had very little, if any, effect on the catalytic properties of the enzyme. Sodium [ RC]lactate has been used to study the conversion of lactate to liver glycogen in the rat. Lactate labelled with C at position 2 and C at position 3 was also used. Carbon-13 was measured by mass spectrometry [187]. Similar experiments were carried out with RC-labelled propionate and C- and C-labelled propionate [188]. 

However, the en2ymes of both pathways have been demonstrated in Candida utilis where xylose isomerase was found inducible and isomerization pathway occurred when xylose as the carbon source was alone in the medium [94, 95]. When both pentoses and hexoses were supplied to the medium, the conversion was carried out through oxido-reductive pathway. Indirect evidence for the presence of xylose isomerase in Rhodotorula gracilis and other yeast hke organisms has also been reported [96, 97]. 

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