Candida lipolytica lipase

PSL Pseudomona sp. lipase, CAL-B Candida antarctica B lipase, CAL Candida antarctica lipase, not specified, CLL Candida lipolytica lipase, SML Serratia marcensens lipase, HLL Humicola lanuginosa lipase). 

Several crude lipase preparations are available from the yeasts Candida lipolytica, C. antarctica (CAL), and C. rugosa (CRL, syn. C. cylindracea). The latter enzyme, the three-dimentional structure of which has been resolved by X-ray analysis [333], has been frequently used for the resolution of esters of secondary alcohols [401-406] and, to a lesser extent, for the resolution of a-substituted carboxylates [407,408]. The CRL preparations from several commercial sources which contain up to 16% of protein [409] differ to some extent in their activity but their selectivity is very similar [410]. As CRL is able to accommodate relatively bulky esters in its active site, it is the lipase of choice for the selective hydrolysis of esters of cyclic secondary alcohols. To illustrate this point, some representative examples are given below. 

Tiiacylglycerol lipase Aspergillus niger, A. oryzae, Candida lipolytica, Mucor javanicus, M. miehei, Rhizopus arrhizus, R. niveus 2,3... 

The mechanism of lipase activity is described under section 3.7.1.1. Lipase from microbial sources (e. g. Candida lipolytica) is utilized for enhancement of aromas in cheesemaking. Limited hydrolysis of milk fat is also of interest in the production of chocolate milk. It enhances the milk character of the flavor. 

Fukumoto et al., 1963), A. oryzae (Arbigeetal., 1986), Geotrichum candidum (Jensen, 1974 Baillargeon and McCarthy, 1991), Candida cylindracea (Benzonana and Esposito, 1971), C. lipolytica (Alifax, 1979), Rhizopus dele-mar (Fukumoto et al., 1964), and Rh. arrhizus (Verhaeghe et al., 1990). The diversity of properties of these lipases, such as pH optimum and, in particular, specificity, enables the selection of an appropriate enzyme for a specific purpose (Kilara, 1985 Fox and Grufferty, 1991). 

---