Candida cylindracea lipase applications

Lara, P. V., and Park, E. Y. 2004. Potential application of waste activated bleaching earth on the production of fatty acid alkyl esters using Candida cylindracea lipase in organic solvent system. Enzyme and Microb. Technol., 34, 270-277. 

Lipase (Candida rugosa formerly Candida cylindracea) Produced as an off-white to tan powder by controlled fermentation using Candida rugosa. Soluble in water but practically insoluble in alcohol, in chloroform, and in ether. Major active principle lipase. Typical applications hydrolysis of lipids, manufacture of dairy products and confectionery goods, development of flavor in processed foods. 

The possible application of enzyme-assisted reactions for production of lower value nonspecialty lipids such as margarine hardstocks and cooking oils has been reported (50). When nonspecific lipases, such as those from Candida cylindraceae and C. antarctica, are used as biocatalysts for interesterification of oil blends, the TAG... 

Microbial lipases from Aspergillus niger (AN), Candida cylindracea (CC), Pseudomonas spp. (PS), Chromobacterium viscosum (CV), Rhizopus delemer (RD), and Rhizopus javanicus (RJ) have been widely used in modifying PUFA-rich oils (117). The fatty acid specificity of lipases (discrimination of PUFA over short-chain fatty acids) is a crucial factor when considering the application of enzymes to modify marine oils rich in PUFA (118-121). Lipases from Rhizopus spp are known to be 1,3-position specific (122). Wanasundara (79) and Wanasundara and Shahidi (123, 124) (seal blubber oil SBO and menhaden oil MHO) and Tanaka et al. (117)... 

Use of Enzymes. The big advantage of enzyme applications is their well-known specificity in reaction pathways. A survey in the biotechnological use of enzymes showed that 65 % of all reported applications fell into the classes of esterolytic reactions (40%) and dehydrogenase reactions (25 %) pig liver esterase, pig pancreatic lipase, the lipase from Candida cylindracea and baker s yeast being the most frequently used biocatalysts (35). For the generation of C 13-aroma volatiles, however, two additional enzyme systems have gained importance, (i) hydrolases and... 

The reported applications of esterolytic applications used esterases (21%), lipases (63%), and proteases (16%). The most often and widely used biocatalysts were pig liver esterase, pig pancreatic lipase and lipase from Candida cylindracea. Among the proteases, a-chymotryp-sin, papain, penicillin aminoacylase and subtilisin were used most frequently. In the case of stereospecific reductions of carbonyl compounds the use of whole cells of baker s yeast (Sac-charomyces cerevisiae) [75-77] accounted for 54% of the total dehydrogenase applications. The dominance of just very few biocatalytic systems, seems to indicate the direction in which... 

As one would expect, microbial enzymes range in specificity. For example, lipases from Candida cylindracea and Staph aureus have little specificity while those from Geotrichum candidum are specific for long chain acids [57], which are of little value in this application. Fortunately, most microbial hpases show specificity for the one and three positions of the triglyceride where the short chain fatty acids are found. While substantial information exists in the literature characterizing lipase enzyme activity to help in enzyme choice [58], enzymes must be evaluated for function in a model system. This often is done in a curd slurry system, which is produced by blending two parts of fresh cheese curd plus one part sterile dilute salt solution (5.2%... 

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